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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- OAAB05379 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- IGH Antibody (C-Term) Affinity Purified Rabbit Polyclonal Antibody (0.1 mg)
- Antibody type
- Polyclonal
- Reactivity
- Human
- Vial size
- 0.1 mg
- Storage
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of IGH Antibody (C-Term) in HL-60, A2058 cell lysates (35ug/lane). IGH (arrow) was detected using the purified Pab.
- Sample type
- HL-60, A2058 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skin reacted with IGH Antibody (C-Term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Sample type
- human skin
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- IGH Antibody (C-Term) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- HL-60 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
