R20187
- ELISA
Supportive data in Antibodypedia
- Immunocytochemistry
Recommended by provider
- Immunohistochemistry
- Recommended by provider
- Flow cytometry
- Recommended by provider
Antibody data
- Product number
- R20187
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Human IgG1 Antibody
- Provider product page
- NSJ Bioreagents - R20187
- Antibody type
- Recombinant
- Description
- Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM117
- Vial size
- 100 µg
- Concentration
- 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant Human IgG1 antibody at -20°C.
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA with human plasma using recombinant Human IgG1 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA with human IgG1 using recombinant Human IgG1 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA of hIgGs shows recombinant Human IgG1 antibody only reacted to hIgG1. No cross reactivity with IgG2, IgG3, IgG4, IgE, IgD, IgA, or mouse/rat/goat IgG.
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA Titration: the plate was coated with different amounts of human IgG1. A serial dilution of recombinant Human IgG1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
