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- Product number
- R20187 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Human IgG1 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant Human IgG1 antibody is suitable for use in Immunocytochemistry/Immunohistochemistry/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM117
- Vial size
- 100 ug
- Concentration
- 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant Human IgG1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA with human plasma using recombinant Human IgG1 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA with human IgG1 using recombinant Human IgG1 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA of hIgGs shows recombinant Human IgG1 antibody only reacted to hIgG1. No cross reactivity with IgG2, IgG3, IgG4, IgE, IgD, IgA, or mouse/rat/goat IgG.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA Titration: the plate was coated with different amounts of human IgG1. A serial dilution of recombinant Human IgG1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
