R20187

antibody from NSJ Bioreagents

Targeting: IGHG1

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

R20187

Provider

NSJ Bioreagents

Product name

Recombinant Human IgG1 Antibody

Antibody type

Recombinant

Description

Purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RM117

Vial size

100 µg

Concentration

1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide

Storage

Store the recombinant Human IgG1 antibody at -20°C.

ELISA

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA with human plasma using recombinant Human IgG1 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Sandwich ELISA with human IgG1 using recombinant Human IgG1 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA of hIgGs shows recombinant Human IgG1 antibody only reacted to hIgG1. No cross reactivity with IgG2, IgG3, IgG4, IgE, IgD, IgA, or mouse/rat/goat IgG.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ELISA Titration: the plate was coated with different amounts of human IgG1. A serial dilution of recombinant Human IgG1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.