10597-1-AP

antibody from Proteintech Group

Targeting: HEY2 bHLHb32, HERP1, HESR2

Provider product page for 10597-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [44]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 10597-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10597-1-AP, RRID:AB_2118415
Product name: HEY2 antibody
Antibody type: Polyclonal
Description: KD/KO validated HEY2 antibody (Cat. #10597-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

HEY2 protein structure - 10597-1-AP shown in red.

Submitted references Villus myofibroblasts are developmental and adult progenitors of mammalian gut lymphatic musculature.

Sanketi BD, Mantri M, Huang L, Tavallaei MA, Hu S, Wang MFZ, De Vlaminck I, Kurpios NA
Developmental cell 2024 May 6;59(9):1159-1174.e5

Identification of signaling pathways that specify a subset of migrating enteric neural crest cells at the wavefront in mouse embryos.

Zhou B, Feng C, Sun S, Chen X, Zhuansun D, Wang D, Yu X, Meng X, Xiao J, Wu L, Wang J, Wang J, Chen K, Li Z, You J, Mao H, Yang S, Zhang J, Jiao C, Li Z, Yu D, Wu X, Zhu T, Yang J, Xiang L, Liu J, Chai T, Shen J, Mao CX, Hu J, Hao X, Xiong B, Zheng S, Liu Z, Feng J
Developmental cell 2024 Jul 8;59(13):1689-1706.e8

MEK/ERK signaling drives the transdifferentiation of supporting cells into functional hair cells by modulating the Notch pathway.

Ma J, Xia M, Guo J, Li W, Sun S, Chen B
Stem cells translational medicine 2024 Jul 15;13(7):661-677

Loss of myocardial Hey2/Hrt2 function disrupts rightward shift of atrioventricular cushion tissue and causes tricuspid atresia.

Okumura K, Ioka T, Sakabe M
Developmental dynamics : an official publication of the American Association of Anatomists 2024 Jan;253(1):107-118

Photobiomodulation (800 nm Light-Emitting Diode) Treatment Promotes Bone Mesenchymal Stem Cell Proliferation Via Long Noncoding RNA MEG3-MicroRNA-217-5P Pathway.

Liu N, Cao L, Peng L, Lu W, Dai X, Wang S, Guo G, Qu X, Xu Y, Zhu C
Photobiomodulation, photomedicine, and laser surgery 2023 Jan;41(1):10-16

Multi-chamber cardioids unravel human heart development and cardiac defects.

Schmidt C, Deyett A, Ilmer T, Haendeler S, Torres Caballero A, Novatchkova M, Netzer MA, Ceci Ginistrelli L, Mancheno Juncosa E, Bhattacharya T, Mujadzic A, Pimpale L, Jahnel SM, Cirigliano M, Reumann D, Tavernini K, Papai N, Hering S, Hofbauer P, Mendjan S
Cell 2023 Dec 7;186(25):5587-5605.e27

Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling.

Shi Y, Zhang D, Chen J, Jiang Q, Song S, Mi Y, Wang T, Ye Q
International journal of biological sciences 2022;18(10):4233-4244

Homocysteine causes neuronal leptin resistance and endoplasmic reticulum stress.

Preninka AI, Kuriya K, Yazawa K, Yoshii M, Yanase Y, Jockers R, Dam J, Hosoi T, Ozawa K
PloS one 2022;17(12):e0278965

Vasorin-containing small extracellular vesicles retard intervertebral disc degeneration utilizing an injectable thermoresponsive delivery system.

Liao Z, Ke W, Liu H, Tong B, Wang K, Feng X, Hua W, Wang B, Song Y, Luo R, Liang H, Zhang W, Zhao K, Li S, Yang C
Journal of nanobiotechnology 2022 Sep 19;20(1):420

Modeling human multi-lineage heart field development with pluripotent stem cells.

Yang D, Gomez-Garcia J, Funakoshi S, Tran T, Fernandes I, Bader GD, Laflamme MA, Keller GM
Cell stem cell 2022 Sep 1;29(9):1382-1401.e8

Notch pathway inhibition mediated by arsenic trioxide depletes tumor initiating cells in small cell lung cancer.

Yang MH, Li B, Chang KJ
Molecular biology reports 2022 Mar;49(3):2245-2253

Notch Signaling Induced by Endoplasmic Reticulum Stress Regulates Cumulus-Oocyte Complex Expansion in Polycystic Ovary Syndrome.

Koike H, Harada M, Kusamoto A, Kunitomi C, Xu Z, Tanaka T, Urata Y, Nose E, Takahashi N, Wada-Hiraike O, Hirota Y, Koga K, Osuga Y
Biomolecules 2022 Jul 27;12(8)

Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells.

Funakoshi S, Fernandes I, Mastikhina O, Wilkinson D, Tran T, Dhahri W, Mazine A, Yang D, Burnett B, Lee J, Protze S, Bader GD, Nunes SS, Laflamme M, Keller G
Nature communications 2021 May 26;12(1):3155

Pseudogene ACTBP2 increases blood-brain barrier permeability by promoting KHDRBS2 transcription through recruitment of KMT2D/WDR5 in Aβ(1-)(42) microenvironment.

Liu Q, Liu X, Zhao D, Ruan X, Su R, Shang X, Wang D, Yang C, Xue Y
Cell death discovery 2021 Jun 14;7(1):142

Evidence that TD-198946 enhances the chondrogenic potential of human synovium-derived stem cells through the NOTCH3 signaling pathway.

Kobayashi M, Chijimatsu R, Hart DA, Hamamoto S, Jacob G, Yano F, Saito T, Shimomura K, Ando W, Chung UI, Tanaka S, Yoshikawa H, Nakamura N
Journal of tissue engineering and regenerative medicine 2021 Feb;15(2):103-115

Extrahepatic cholangiocyte obstruction is mediated by decreased glutathione, Wnt and Notch signaling pathways in a toxic model of biliary atresia.

Fried S, Gilboa D, Har-Zahav A, Lavrut PM, Du Y, Karjoo S, Russo P, Shamir R, Wells RG, Waisbourd-Zinman O
Scientific reports 2020 May 5;10(1):7599

Development of Human CBF1-Targeting Single-Stranded DNA Aptamers with Antiangiogenic Activity In Vitro.

Tezuka-Kagajo M, Maekawa M, Ogawa A, Hatta Y, Ishii E, Eguchi M, Higashiyama S
Nucleic acid therapeutics 2020 Dec;30(6):365-378

TMEM100 is a key factor for specification of lymphatic endothelial progenitors.

Moon EH, Kim YH, Vu PN, Yoo H, Hong K, Lee YJ, Oh SP
Angiogenesis 2020 Aug;23(3):339-355

Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts.

Gage BK, Liu JC, Innes BT, MacParland SA, McGilvray ID, Bader GD, Keller GM
Cell stem cell 2020 Aug 6;27(2):254-269.e9

Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis.

Quilichini E, Fabre M, Dirami T, Stedman A, De Vas M, Ozguc O, Pasek RC, Cereghini S, Morillon L, Guerra C, Couvelard A, Gannon M, Haumaitre C
Cellular and molecular gastroenterology and hepatology 2019;8(3):487-511

Placental P-glycoprotein inhibition enhances susceptibility to Di-(2-ethylhexyl)-phthalate induced cardiac malformations in mice: A possibly promising target for congenital heart defects prevention.

Tang C, Luo C, Hua Y, Zhou K, Duan H, Ma F, Zhang Y, Li Y, Qiu D, Wang C
PloS one 2019;14(5):e0214873

JAG2/Notch2 inhibits intervertebral disc degeneration by modulating cell proliferation, apoptosis, and extracellular matrix.

Long J, Wang X, Du X, Pan H, Wang J, Li Z, Liu H, Li X, Zheng Z
Arthritis research & therapy 2019 Oct 16;21(1):213

Three-dimensional live imaging of Atoh1 reveals the dynamics of hair cell induction and organization in the developing cochlea.

Tateya T, Sakamoto S, Ishidate F, Hirashima T, Imayoshi I, Kageyama R
Development (Cambridge, England) 2019 Nov 1;146(21)

MicroRNA-98 reduces amyloid β-protein production and improves oxidative stress and mitochondrial dysfunction through the Notch signaling pathway via HEY2 in Alzheimer's disease mice.

Chen FZ, Zhao Y, Chen HZ
International journal of molecular medicine 2019 Jan;43(1):91-102

Notch1 regulates endothelial apoptosis via the ERK pathway in chronic obstructive pulmonary disease.

Zong D, Li J, Cai S, He S, Liu Q, Jiang J, Chen S, Long Y, Chen Y, Chen P, Ouyang R
American journal of physiology. Cell physiology 2018 Sep 1;315(3):C330-C340

Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis.

Churko JM, Garg P, Treutlein B, Venkatasubramanian M, Wu H, Lee J, Wessells QN, Chen SY, Chen WY, Chetal K, Mantalas G, Neff N, Jabart E, Sharma A, Nolan GP, Salomonis N, Wu JC
Nature communications 2018 Nov 21;9(1):4906

The effect of maternal exposure to di-(2-ethylhexyl)-phthalate on fetal cardiac development in mice.

Tang C, Deng Y, Duan H, Zhang Y, Li Y, Qiu D, Zhou K, Hua Y, Wang C
Journal of applied toxicology : JAT 2018 Jun;38(6):834-842

Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.

Qiu GZ, Mao XY, Ma Y, Gao XC, Wang Z, Jin MZ, Sun W, Zou YX, Lin J, Fu HL, Jin WL
Cancer science 2018 Jul;109(7):2199-2210

miR-150 enhances apoptotic and anti-tumor effects of paclitaxel in paclitaxel-resistant ovarian cancer cells by targeting Notch3.

Kim TH, Jeong JY, Park JY, Kim SW, Heo JH, Kang H, Kim G, An HJ
Oncotarget 2017 Sep 22;8(42):72788-72800

MicroRNA-136 inhibits cancer stem cell activity and enhances the anti-tumor effect of paclitaxel against chemoresistant ovarian cancer cells by targeting Notch3.

Jeong JY, Kang H, Kim TH, Kim G, Heo JH, Kwon AY, Kim S, Jung SG, An HJ
Cancer letters 2017 Feb 1;386:168-178

Cigarette smoke enhances initiation and progression of lung cancer by mutating Notch1/2 and dysregulating downstream signaling molecules.

Li W, Zhou J, Chen Y, Zhang G, Jiang P, Hong L, Shen Y, Wang X, Gong X
Oncotarget 2017 Dec 29;8(70):115128-115139

Notch3-specific inhibition using siRNA knockdown or GSI sensitizes paclitaxel-resistant ovarian cancer cells.

Kang H, Jeong JY, Song JY, Kim TH, Kim G, Huh JH, Kwon AY, Jung SG, An HJ
Molecular carcinogenesis 2016 Jul;55(7):1196-209

Pharyngeal arch artery defects and lethal malformations of the aortic arch and its branches in mice deficient for the Hrt1/Hey1 transcription factor.

Fujita M, Sakabe M, Ioka T, Watanabe Y, Kinugasa-Katayama Y, Tsuchihashi T, Utset MF, Yamagishi H, Nakagawa O
Mechanisms of development 2016 Feb;139:65-73

Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination.

Wu LM, Wang J, Conidi A, Zhao C, Wang H, Ford Z, Zhang L, Zweier C, Ayee BG, Maurel P, Zwijsen A, Chan JR, Jankowski MP, Huylebroeck D, Lu QR
Nature neuroscience 2016 Aug;19(8):1060-72

EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway.

Xie H, Sun J, Chen Y, Zong M, Li S, Wang Y
Oxidative medicine and cellular longevity 2015;2015:214836

DEHP exposure impairs mouse oocyte cyst breakdown and primordial follicle assembly through estrogen receptor-dependent and independent mechanisms.

Mu X, Liao X, Chen X, Li Y, Wang M, Shen C, Zhang X, Wang Y, Liu X, He J
Journal of hazardous materials 2015 Nov 15;298:232-40

An important role of endothelial hairy-related transcription factors in mouse vascular development.

Morioka T, Sakabe M, Ioka T, Iguchi T, Mizuta K, Hattammaru M, Sakai C, Itoh M, Sato GE, Hashimoto A, Fujita M, Okumura K, Araki M, Xin M, Pedersen RA, Utset MF, Kimura H, Nakagawa O
Genesis (New York, N.Y. : 2000) 2014 Nov;52(11):897-906

Cathepsin K-mediated Notch1 activation contributes to neovascularization in response to hypoxia.

Jiang H, Cheng XW, Shi GP, Hu L, Inoue A, Yamamura Y, Wu H, Takeshita K, Li X, Huang Z, Song H, Asai M, Hao CN, Unno K, Koike T, Oshida Y, Okumura K, Murohara T, Kuzuya M
Nature communications 2014 Jun 4;5:3838

Notch promotes vascular maturation by inducing integrin-mediated smooth muscle cell adhesion to the endothelial basement membrane.

Scheppke L, Murphy EA, Zarpellon A, Hofmann JJ, Merkulova A, Shields DJ, Weis SM, Byzova TV, Ruggeri ZM, Iruela-Arispe ML, Cheresh DA
Blood 2012 Mar 1;119(9):2149-58

Genetic modifiers predisposing to congenital heart disease in the sensitized Down syndrome population.

Li H, Cherry S, Klinedinst D, DeLeon V, Redig J, Reshey B, Chin MT, Sherman SL, Maslen CL, Reeves RH
Circulation. Cardiovascular genetics 2012 Jun;5(3):301-8

Tmem100, an ALK1 receptor signaling-dependent gene essential for arterial endothelium differentiation and vascular morphogenesis.

Somekawa S, Imagawa K, Hayashi H, Sakabe M, Ioka T, Sato GE, Inada K, Iwamoto T, Mori T, Uemura S, Nakagawa O, Saito Y
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 24;109(30):12064-9

TAp73 acts via the bHLH Hey2 to promote long-term maintenance of neural precursors.

Fujitani M, Cancino GI, Dugani CB, Weaver IC, Gauthier-Fisher A, Paquin A, Mak TW, Wojtowicz MJ, Miller FD, Kaplan DR
Current biology : CB 2010 Nov 23;20(22):2058-65

Id4, a new candidate gene for senile osteoporosis, acts as a molecular switch promoting osteoblast differentiation.

Tokuzawa Y, Yagi K, Yamashita Y, Nakachi Y, Nikaido I, Bono H, Ninomiya Y, Kanesaki-Yatsuka Y, Akita M, Motegi H, Wakana S, Noda T, Sablitzky F, Arai S, Kurokawa R, Fukuda T, Katagiri T, Schönbach C, Suda T, Mizuno Y, Okazaki Y
PLoS genetics 2010 Jul 8;6(7):e1001019

Rbpj conditional knockout reveals distinct functions of Notch4/Int3 in mammary gland development and tumorigenesis.

Raafat A, Lawson S, Bargo S, Klauzinska M, Strizzi L, Goldhar AS, Buono K, Salomon D, Vonderhaar BK, Callahan R
Oncogene 2009 Jan 15;28(2):219-30

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP(HEY2 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using HEY2 antibody 10597-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-HEY2 (IP:10597-1-AP, 5ug; Detection:10597-1-AP 1:500) with mouse heart tissue lysate 7500ug.
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human prostate cancer using 10597-1-AP(HEY2 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue