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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Protein array [1]
- Other assay [1]
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- Product number
- V4991 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Lactoferrin Antibody / Lactotransferrin / LTF
- Antibody type
- Monoclonal
- Description
- This highly specific Lactoferrin antibody is suitable for use in Flow cytometry/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- LTF/4072
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Aliquot the Lactoferrin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast carcinoma tissue with Lactoferrin antibody (clone LTF/4072). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of PFA-fixed human MCF-7 cells with Lactoferrin antibody (clone LTF/4072) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using Lactoferrin antibody (clone LTF/4072). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to be specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free Lactoferrin antibody (clone LTF/4072) as confirmation of integrity and purity.
