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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29744 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Lactoferrin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: human milk.
- Concentration
- 0.2 mg/mL
Submitted references NHBA is processed by kallikrein from human saliva.
Oxidative Injury and Iron Redistribution Are Pathological Hallmarks of Marmoset Experimental Autoimmune Encephalomyelitis.
Pantano E, Marchi S, Biagini M, Di Fede M, Nardi Dei V, Rossi Paccani S, Pizza M, Cartocci E
PloS one 2019;14(8):e0203234
PloS one 2019;14(8):e0203234
Oxidative Injury and Iron Redistribution Are Pathological Hallmarks of Marmoset Experimental Autoimmune Encephalomyelitis.
Dunham J, Bauer J, Campbell GR, Mahad DJ, van Driel N, van der Pol SMA, 't Hart BA, Lassmann H, Laman JD, van Horssen J, Kap YS
Journal of neuropathology and experimental neurology 2017 Jun 1;76(6):467-478
Journal of neuropathology and experimental neurology 2017 Jun 1;76(6):467-478
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Lactoferrin using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Lactoferrin polyclonal antibody (Product # PA5-29744) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Lactoferrin was performed by separating 5 µg of Human milk by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Lactoferrin Polyclonal Antibody (Product # PA5-29744) at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Lactoferrin Polyclonal Antibody (Product # PA5-29744). Human milk (5 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lactoferrin Polyclonal Antibody (Product # PA5-29744) diluted at 1:20,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using Lactoferrin (Product # PA5-29744) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 Lactoferrin in Saliva fractions and their activity on NHBA cleavage. A) SDS PAGE of the fractions obtained through the saliva partial purification incubated with NHBA. All the samples were maintained at 37degC overnight and then loaded on SDS-PAGE. In the first four lanes, NHBA recombinant protein, saliva, saliva post dialysis and saliva post dialysis incubated with NHBA as reported as control. From number 2 to number 64, are reported the different fractions obtained from saliva purification after incubation with NHBA recombinant protein. NHBA at a concentration of 1 mg/ml was incubated with the same volume of each fraction. B) Immunodetection on saliva recovered fractions against Lactoferrin. Recombinant Lactoferrin (rLF), saliva (saliva) and the fractions from AEC were loaded on the gel. Lactoferrin is present in frations from 34-37, 48-50 and 59-60.
