Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB4524 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4524, RRID:AB_1580070
- Product name
- RPS6KA4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RPS6KA4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Dynamic changes in histone H3 phosphoacetylation during early embryonic stem cell differentiation are directly mediated by mitogen- and stress-activated protein kinase 1 via activation of MAPK pathways.
Lee ER, McCool KW, Murdoch FE, Fritsch MK
The Journal of biological chemistry 2006 Jul 28;281(30):21162-72
The Journal of biological chemistry 2006 Jul 28;281(30):21162-72
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The RPS6KA4 polyclonal antibody (Cat # PAB4524) is used in Western blot to detect RPS6KA4 in placenta tissue lysate .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Indirect immunofluorescence analysis showed that RPS6KA4 is predominantly localized in the nucleus of parental (10T1/2) and oncogene-transformed (Ciras-3) mouse fibroblasts . Cells were co-stained with DAPI to visualize nucleus compartment . Data is kindly provided by B . Drobic and Dr . J . Davie from University of Manitoba (Winnipeg, Canada) .
- Validation comment
- Immunofluorescence