Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB2310 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse MSK2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse MSK2 in Western blots. Does not detect recombinant human MSK1.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
O75676
- Isotype
- IgG
- Antibody clone number
- 261034
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mitogen- and stress-activated protein kinase 2 and cyclic AMP response element binding protein are activated in lesional psoriatic epidermis.
Funding AT, Johansen C, Kragballe K, Iversen L
The Journal of investigative dermatology 2007 Aug;127(8):2012-9
The Journal of investigative dermatology 2007 Aug;127(8):2012-9
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse MSK2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse MSK2 Monoclonal Antibody (Catalog # MAB2310) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human MSK1 and MSK2 (2 ng/lane) were included. A specific band for MSK2 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.