PAB22086

antibody from Abnova Corporation

Targeting: HINT3 FLJ33126, HINT4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22086

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22086, RRID:AB_10963778

Product name

HINT3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant HINT3.

Antigen sequence

MAEEQVNRSAGLAPDCEASATAETTVSSVGTCEAA
AKSPEPKDYDSTCVFCRIAGRQDPGTELLHCENED
LICFKDIKPAATHHYLVVP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with HINT3 polyclonal antibody (Cat # PAB22086) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with HINT3 polyclonal antibody (Cat # PAB22086) at 1-4 ug/mL dilution shows positivity in cytoplasm and mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with HINT3 polyclonal antibody (Cat # PAB22086) shows moderate cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)