R30934

antibody from NSJ Bioreagents

Targeting: PRDX3 AOP-1, AOP1, MER5, SP-22

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R30934

Provider

NSJ Bioreagents

Product name

Peroxiredoxin 3 Antibody

Antibody type

Polyclonal

Description

This highly specific Peroxiredoxin 3 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Peroxiredoxin 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Peroxiredoxin 3 antibody and Lane 1: rat brain; 2: (r) lung; 3: (r) kidney; and human cell line lysate 4: HeLa; 5: Jurkat; 6: 293T; 7: MCF-7; 8: A549; 9: U20S. Epected size ~28KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human MCF7, 3) rat brain, 4) rat liver, 5) mouse brain and 6) mouse liver tissue lysate with Peroxiredoxin 3 antibody. Predicted molecular weight ~28 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human A549 cells with Peroxiredoxin 3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Peroxiredoxin 3 antibody testing of human breast cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Peroxiredoxin 3 antibody testing of rat intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Peroxiredoxin 3 antibody testing of A549 cells

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human breast cancer tissue with Peroxiredoxin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat intestine tissue with Peroxiredoxin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HeLa cells with Peroxiredoxin 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Peroxiredoxin 3 antibody.