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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- R30933 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Peroxiredoxin 3 Antibody / PRDX3
- Antibody type
- Polyclonal
- Description
- This highly specific Peroxiredoxin 3 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- The lyophilized Peroxiredoxin 3 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Peroxiredoxin 3 antibody and rat samples: 1. brain, 2. lung, 3. liver, 4. kidney; and human samples: 5. HeLa, 6. Jurkat, 7. 293T, 8. MCF-7, 9. A549. Expected size: ~28KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human A549, 3) human HEK293, 4) human MCF7, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with Peroxiredoxin 3 antibody. Predicted molecular weight ~28 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of Peroxiredoxin 3 antibody and A549 cells
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A549 cells with Peroxiredoxin 3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer tissue with Peroxiredoxin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Peroxiredoxin 3 antibody staining of rat intestine tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat intestine tissue with Peroxiredoxin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
