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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab121327 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Peroxiredoxin 3/PRDX3 Antibody
- Antibody type
- Recombinant
- Description
- MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.Rabbit anti Human Peroxiredoxin 3/PRDX3 Antibody, Recombinant, could be used for WB, ICC, IF, IHC and so on.ApplicationWB: 1/500-1/1000ICC/IF: 1/50-1/200IHC: 1/50-1/100Protein Function Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 243-256- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,2 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) - Western Blot All lanes: Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 23 kDa Exposure time: 8.6 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) - IF/ICC Immunocytochemistry analysis of Peroxiredoxin 3 (green) in HeLa using Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant Peroxiredoxin 3/PRDX3 Antibody (Ab121327). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
