Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP12100c - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP12100c, RRID:AB_10838597
- Product name
- PRDX4 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.25 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- PRDX4 Antibody (Center) (Cat. #AP12100c) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PRDX4 antibody detected the PRDX4 protein (arrow).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of PRDX4 (arrow) using rabbit polyclonal PRDX4 Antibody (Center) (Cat. #AP12100c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX4 gene.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- PRDX4 Antibody (Center) (Cat. #AP12100c) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the PRDX4 antibody detected the PRDX4 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- PRDX4 Antibody (Center) (Cat. #AP12100c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRDX4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100