Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- MAB9186-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TSPAN1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TSPAN1 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O60635
- Isotype
- IgG
- Antibody clone number
- 819202
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TSPAN1 in HEK293 Human Cell Line Transfected with Human TSPAN1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TSPAN1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN1 Monoclonal Antibody (Catalog # MAB9186) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB007). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TSPAN1 in HT-29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TSPAN1 Monoclonal Antibody (Catalog # MAB9186, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.