Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-82440 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSPAN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TMAEHFLTLL VVPAIKKDYG SQEDFTQVWN TTMKGLKCCG FTNYTDFEDS PYFKENSAFP PFCCNDNVTN TANETCTKQK AHDQKVEGCF NQLLYDIRTN AVTV
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TSPAN1 by a TSPAN1 polyclonal antibody (Product # PA5-82440). Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TSPAN1 in A-431 cells using a TSPAN1 polyclonal antibody (Product # PA5-82440). The analysis shows localization to nucleoplasm & vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TSPAN1 in human kidney and skeletal muscle tissues using a TSPAN1 polyclonal antibody (Product # PA5-82440). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TSPAN1 in human colon using a TSPAN1 polyclonal antibody (Product # PA5-82440). The analysis shows weak to moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TSPAN1 in human prostate using a TSPAN1 polyclonal antibody (Product # PA5-82440). The analysis shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TSPAN1 in human kidney using a TSPAN1 polyclonal antibody (Product # PA5-82440). The analysis shows moderate to strong cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TSPAN1 in human skeletal muscle using a TSPAN1 polyclonal antibody (Product # PA5-82440). The analysis shows no positivity in myocytes as expected.