PA5-100281

POMGNT1 antibody from Invitrogen Antibodies

FLJ20277, LGMD2O, MEB, MGAT1.2

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Provider product page for PA5-100281

Antibody Data

Product number

PA5-100281

Provider

Invitrogen Antibodies

Product name

POMGNT1 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POMGNT1 in HepG2 cell lysate (left lane: treated with blocking peptide). Samples were incubated with POMGNT1 polyclonal antibody (Product # PA5-100281).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POMGNT1 in myeloma cells lysate (left lane: treated with blocking peptide). Samples were incubated with POMGNT1 polyclonal antibody (Product # PA5-100281).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POMGNT1 in HepG2 cell lysate (left lane: treated with blocking peptide). Samples were incubated with POMGNT1 polyclonal antibody (Product # PA5-100281).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POMGNT1 in myeloma cells lysate (left lane: treated with blocking peptide). Samples were incubated with POMGNT1 polyclonal antibody (Product # PA5-100281).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of POMGNT1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and POMGNT1 polyclonal antibody (Product # PA5-100281) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).