LF-MA0002

antibody from Invitrogen Antibodies

Targeting: PRDX5 ACR1, AOEB166, B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

LF-MA0002

Provider

Invitrogen Antibodies

Product name

Anti-PRDX5 Monoclonal Antibody (3F11)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

A suggested positive control for this product is HeLa cells.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

3F11

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Dimeric peroxiredoxins are druggable targets in human Burkitt lymphoma.

Trzeciecka A, Klossowski S, Bajor M, Zagozdzon R, Gaj P, Muchowicz A, Malinowska A, Czerwoniec A, Barankiewicz J, Domagala A, Chlebowska J, Prochorec-Sobieszek M, Winiarska M, Ostaszewski R, Gwizdalska I, Golab J, Nowis D, Firczuk M
Oncotarget 2016 Jan 12;7(2):1717-31

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of LNCaP (Lane 1), U-87 MG (Lane 2), HeLa (Lane 3), NTERA-2 (Lane 4), F9 (Lane 5) and tissue extract (30 µg lysate) of Mouse Kidney (Lane 6). The blot was probed with Anti-PRDX5 Mouse Monoclonal Antibody (Product # LF-MA0002, 1:1,000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 17 kDa band corresponding to PRDX5 was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of PRDX5 was done on LNCap cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PRDX5 Mouse Monoclonal Antibody (Product # LF-MA0002, red histogram) at 1:20 dilution or a matched isotype control (pink histogram) in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature. A representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.