ARG54703

antibody from Arigo

Targeting: EZH2 ENX-1, EZH1, KMT6, KMT6A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG54703

Provider

Arigo

Product name

anti-EZH2 antibody

Antibody type

Polyclonal

Antigen

Human EZH2 recombinant protein (NP_001190176.1).

Description

Protein A purified

Reactivity

Human, Mouse

Host

Rabbit

Vial size

200 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 20 µg of MDA-MB-468, SW620, T47D cell line, mouse spleen, mouse testis tissue lysates (from left to right) stained with ARG54703 anti-EZH2 antibody at 1:1000 dilution.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 20 µg of lysates from Jurkat, T47D cell line (from left to right) stained with ARG54703 anti-EZH2 antibody at 1:2000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: 293 cell stained with ARG54703 anti-EZH2 antibody. Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: human testis tissue stained with ARG54703 anti-EZH2 antibody.

Flow cytometry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Flow Cytometry: Jurkat cells stained with ARG54703 anti-EZH2 antibody (bottom histogram) or without primary antibody control (top histogram).

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Flow Cytometry: HeLa cells stained with ARG54703 anti-EZH2 antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.