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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX82503 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX82503, RRID:AB_11167802
- Product name
- EZH2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references EZH2, Endothelin-1, and CD34 as Biomarkers of Aggressive Cervical Squamous Cell Carcinoma: An Immunohistochemical Study.
EZH2 inhibitors transcriptionally upregulate cytotoxic autophagy and cytoprotective unfolded protein response in human colorectal cancer cells.
Fathy A, Abdelrahman AE
Turk patoloji dergisi 2018;34(2):150-57
Turk patoloji dergisi 2018;34(2):150-57
EZH2 inhibitors transcriptionally upregulate cytotoxic autophagy and cytoprotective unfolded protein response in human colorectal cancer cells.
Hsieh YY, Lo HL, Yang PM
American journal of cancer research 2016;6(8):1661-80
American journal of cancer research 2016;6(8):1661-80
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of EZH2 Antibody (GTX82503) in MDA-MB231(lane 1), Jurkat(lane 2), MDA-MB468(lane 3) cell line lysates (35μg/lane). EZH2 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of EZH2 Antibody(GTX82503) with 293 cell followed by Alexa Fluor 488-conjμgated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with EZH2 Antibody(GTX82503), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EZH2 Antibody (GTX82503) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
