F48073

antibody from NSJ Bioreagents

Targeting: EZH2 ENX-1, EZH1, KMT6, KMT6A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F48073

Provider

NSJ Bioreagents

Product name

EZH2 Antibody

Antibody type

Polyclonal

Description

This highly specific EZH2 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the EZH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of EZH2 antibody at 1:2000 dilution. Lane 1: Jurkat lysate; 2: MDA-MB-231 lysate; 3: MDA-MB-468 lysate; 4: T47D lysate; 5: mouse spleen lysate; Predicted size: 85-95 kDa

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from (1) MDA-MB-468, (2) SW620, (3) T47D cell line, (4) mouse spleen, (5) mouse testis tissue using EZH2 antibody at 1:1000. Predicted size: 85-95 kDa

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of EZH2 antibody and 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of EZH2 antibody and human HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human testis tissue tested with EZH2 antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using EZH2 antibody (green) compared to an isotype control of rabbit IgG (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

EZH2 antibody flow cytometric analysis of Jurkat cells (bottom histogram) compared to negative control cells (top histogram).