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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- F48073 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EZH2 Antibody
- Antibody type
- Polyclonal
- Antigen
- This EZH2 antibody was produced from rabbits immunized with a recombinant protein from human EZH2.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the EZH2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of EZH2 antibody at 1:2000 dilution. Lane 1: Jurkat lysate; 2: MDA-MB-231 lysate; 3: MDA-MB-468 lysate; 4: T47D lysate; 5: mouse spleen lysate; Predicted size: 85-95 kDa
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from (1) MDA-MB-468, (2) SW620, (3) T47D cell line, (4) mouse spleen, (5) mouse testis tissue using EZH2 antibody at 1:1000. Predicted size: 85-95 kDa
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of EZH2 antibody and 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of EZH2 antibody and human HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue tested with EZH2 antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using EZH2 antibody (green) compared to an isotype control of rabbit IgG (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- EZH2 antibody flow cytometric analysis of Jurkat cells (bottom histogram) compared to negative control cells (top histogram).
