MA5-27661

antibody from Invitrogen Antibodies

Targeting: SOD3 EC-SOD

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27661

Provider

Invitrogen Antibodies

Product name

SOD3 Monoclonal Antibody (4GG11G6)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

1 µg/mL of MA5-27661 was sufficient for detection of EC-SOD in 20 µg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects extracellular SOD approximately 35kDa.

Reactivity

Human, Mouse, Rat, Guinea Pig

Host

Mouse

Isotype

IgG

Antibody clone number

4GG11G6

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SOD3 in human cartilage lysates. The sample was incubated with SOD3 monoclonal antibody (Product # MA5-27661) using a dilution of 1:1000. Samples were arranged as follows: Left) Control, Middle) Young cartilage, Right) Cartilage sample with osteoarthritis.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Extracellular superoxide dismutase [Cu-Zn] was performed using 70% confluent log phase T-47D and Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SOD3 Monoclonal Antibody (4GG11G6) (Product # MA5-27661) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization in T-47D but not in Hep G2 (Panel e) which is reported to be low to negative for SOD3 expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SOD3 in mouse human cartilage. Sample was incubated with SOD3 monoclonal antibody (Product # MA5-27661) using a dilution of 1:1000. Images are shown as follows: Left: control, middle: young cartilage, right: cartilage with osteoarthritis.