RQ6518

antibody from NSJ Bioreagents

Targeting: SOD3 EC-SOD

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6518

Provider

NSJ Bioreagents

Product name

Superoxide dismutase 3 Antibody / Sod3

Antibody type

Polyclonal

Description

This highly specific Superoxide dismutase 3 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with mouse samples.

Reactivity

Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Superoxide dismutase 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse lung and 2) mouse kidney tissue lysate with Superoxide dismutase 3 antibody. Predicted molecular weight: 26~32 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse lung tissue with Superoxide dismutase 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse HEPA1-6 cells with Superoxide dismutase 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Superoxide dismutase 3 antibody.