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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF3420 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SOD3/EC-SOD Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SOD3/EC-SOD in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) SOD1 or rhSOD2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08294- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references SOD3 improves the tumor response to chemotherapy by stabilizing endothelial HIF-2α.
Mira E, Carmona-Rodríguez L, Pérez-Villamil B, Casas J, Fernández-Aceñero MJ, Martínez-Rey D, Martín-González P, Heras-Murillo I, Paz-Cabezas M, Tardáguila M, Oury TD, Martín-Puig S, Lacalle RA, Fabriás G, Díaz-Rubio E, Mañes S
Nature communications 2018 Feb 8;9(1):575
Nature communications 2018 Feb 8;9(1):575
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SOD3/EC-SOD by Western Blot. Western blot shows lysates of human aorta tissue. PVDF Membrane was probed with 0.2 µg/mL of Goat Anti-Human SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOD3/EC-SOD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SOD3/EC-SOD by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for SOD3/EC-SOD at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3420) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SOD3/EC-SOD in Human Kidney. SOD3/EC-SOD was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3420) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
