ANR-132-200UL

antibody from Invitrogen Antibodies

Targeting: SORL1 C11orf32, gp250, LR11, LRP9, SorLA, SorLA-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ANR-132-200UL

Provider

Invitrogen Antibodies

Product name

SORL1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 6), human Colo-205 colon adenocarcinoma cell line lysate (lanes 3 and 7) and HepG2 hepatocellular carcinoma (lanes 4 and 8): - 1-4. Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:200). 5-8. Anti-SORL1 (extracellular) Antibody , preincubated with SORL1 (extracellular) Blocking Peptide (#BLP-NR132).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1, 2. Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:200). 3, 4. Anti-SORL1 (extracellular) Antibody , preincubated with SORL1 (extracellular) Blocking Peptide (#BLP-NR132).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 6), human Colo-205 colon adenocarcinoma cell line lysate (lanes 3 and 7) and HepG2 hepatocellular carcinoma (lanes 4 and 8): - 1-4. Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:200). 5-8. Anti-SORL1 (extracellular) Antibody , preincubated with SORL1 (extracellular) Blocking Peptide (#BLP-NR132).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of SORL1 in mouse hippocampus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in mouse hippocampal CA1 region revealed SORL1 immunoreactivity (green) in neuronal profiles in the pyramidal layer (P) and in the stratum oriens (SO, arrows). B. Pre-incubation of the Antibody with SORL1 (extracellular) Blocking Peptide (BLP-NR132), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of SORL1 in rat parietal cortex. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. SORL1 immunoreactivity (green) appears in neuronal profiles in layer III (arrows). B. Pre-incubation of the Antibody with SORL1 (extracellular) Blocking Peptide (BLP-NR132), suppressed staining. Cell nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of SORL1 in mouse hippocampus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in mouse hippocampal CA1 region revealed SORL1 immunoreactivity (green) in neuronal profiles in the pyramidal layer (P) and in the stratum oriens (SO, arrows). B. Pre-incubation of the Antibody with SORL1 (extracellular) Blocking Peptide (BLP-NR132), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of SORL1 in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-SORL1 (extracellular) Antibody (#ANR-132), (5 µg) + goat- Anti-rabbit-FITC.