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LearnMA5-15355
antibody from Invitrogen Antibodies
Targeting: SORL1
C11orf32, gp250, LR11, LRP9, SorLA, SorLA-1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [3]
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- Product number
- MA5-15355 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SORL1 Monoclonal Antibody (3B6B11)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15355 targets SORL1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15355 immunogen is purified recombinant fragment of human SORL1 expressed in E. Coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3B6B11
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SORL1 using a SORL1 monoclonal antibody (Product # MA5-15355) against a truncated SORL1 recombinant protein.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PANC-1 cells using SORL1 monoclonal antibody (Product # MA5-15355) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PANC-1 cells using SORL1 monoclonal antibody (Product # MA5-15355) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PANC-1 cells using SORL1 monoclonal antibody (Product # MA5-15355) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of SORL1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR840164_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on wild type HeLa (panel a,d), HeLa Cas9 control cells (panel b,e) and HeLa SORL1 KO cells (panel c, f). Cells were fixed, permeabilized, and labeled with SORL1 Monoclonal Antibody (3B6B11) (Product # MA5-15355, 1:100), followed by Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32723, 1:2,000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Loss of signal (panel c,f) upon CRISPR mediated knockout (KO) confirms that antibody is specific to SORL1 (green). The images were captured at 40X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human cerebrum tissues using SORL1 monoclonal antibody (Product # MA5-15355) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain, cortex tissues using SORL1 monoclonal antibody (Product # MA5-15355).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human cerebrum tissues using SORL1 monoclonal antibody (Product # MA5-15355) followed with DAB staining.
