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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
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- Product number
- PA5-140975 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSUN4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NSUN4 in different cell lysates. Samples were incubated in NSUN4 Polyclonal antibody (Product # PA5-140975) using a dilution of 1:1,000. Positive control: Lane 1: MCF-7 ; Lane 2: 293.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of NSUN4 in A549 cells (green). Samples were incubated in NSUN4 Polyclonal antibody (Product # PA5-140975). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of NSUN4 in HepG2 cells (green). Samples were incubated in NSUN4 Polyclonal antibody (Product # PA5-140975). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of NSUN4 in MCF-7 cells (green). Samples were incubated in NSUN4 Polyclonal antibody (Product # PA5-140975). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
