Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP1-51909 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-51909, RRID:AB_11028413
- Product name
- Goat Polyclonal PRPF31 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-LKVKGEKSGLMST
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Protein 61K, encoded by a gene (PRPF31) linked to autosomal dominant retinitis pigmentosa, is required for U4/U6*U5 tri-snRNP formation and pre-mRNA splicing.
Makarova OV, Makarov EM, Liu S, Vornlocher HP, Lührmann R
The EMBO journal 2002 Mar 1;21(5):1148-57
The EMBO journal 2002 Mar 1;21(5):1148-57
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PRPF31 Antibody [NBP1-51909] - (0.3ug/ml) staining of cell line A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PRPF31 Antibody [NBP1-51909] - Analysis of PRPF31 in cell line A431 lysate using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.