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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-89461 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFB4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MSFPKYKPSS LRTLPETLDP AEYNISPETR RAQAERLAIR AQLKREYLLQ YNDPNRRGLI ENPALLRWAY ARTINVYPNF RPTPKNSLMG; Positive Samples: U-87MG, HeLa, 293T, Mouse heart, Mouse kidney, Mouse liver; Cellular Location: Matrix side, Mitochondrion inner membrane, Single-pass membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.96 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NDUFB4 was performed in L929 cells using NDUFB4 Polyclonal Antibody (Product # PA5-89461) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NDUFB4 was performed in U-2 OS cells using NDUFB4 Polyclonal Antibody (Product # PA5-89461) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NDUFB4 in paraffin-embedded mouse kidney. Samples were incubated with NDUFB4 Polyclonal antibody (Product # PA5-89461) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NDUFB4 in paraffin-embedded rat kidney. Samples were incubated with NDUFB4 Polyclonal antibody (Product # PA5-89461) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NDUFB4 in paraffin-embedded human liver. Samples were incubated with NDUFB4 Polyclonal antibody (Product # PA5-89461) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
