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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Other assay [1]
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Validation data
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- Product number
- H00003081-M11 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HGD Monoclonal Antibody (1F1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: MAELKYISGF GNECSSEDPR CPGSLPEGQN NPQVCPYNLY AEQLSGSAFT CPRSTNKRSW LYRILPSVSH KPFESIDEGH VTHNWDEVDP DPNQLRWKPF EIPKASQKKV DFVSGLHTLC GAGDIKSNNG LAIHIFLCNT SMENRCFYNS DGDFLIVPQK GNLLIYTEFG KMLVQPNEIC VIQRGMRFSI DVFEETRGYI LEVYGVHLEL PDLGPIGANG LANPRDFLIP IAWYEDRQVP GGYTVINKYQ GKLFAAKQDV SPFNVVAWHG NYTPYKYNLK NFMVINSVAF DHADPSIFTV LTALRRPARS SWHLRGLPMA PWHLCLNHL
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F1
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HGD expression in transfected 293T cell line by HGD monoclonal antibody (M11), clone 1F1.Lane 1: HGD transfected lysate(50 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HGD is 3 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HGD transfected lysate using anti-HGD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HGD MaxPab rabbit polyclonal antibody.
