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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ6996 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Homogentisate 1,2-dioxygenase Antibody / HGD
- Antibody type
- Monoclonal
- Description
- This highly specific Homogentisate 1,2-dioxygenase antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 2F11E1
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Homogentisate 1,2-dioxygenase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HCCP cell lysate with Homogentisate 1,2-dioxygenase antibody. Predicted molecular weight ~50 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer tissue with Homogentisate 1,2-dioxygenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human Caco-2 cells with Homogentisate 1,2-dioxygenase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Homogentisate 1,2-dioxygenase antibody.
