MA5-14928

antibody from Invitrogen Antibodies

Targeting: MKNK1 MNK1

Antibody data

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Validation data

MKNK1 protein structure - MA5-14928 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of MNK1 was achieved by transfecting HEK 293 cells with MNK1 specific siRNAs (Silencer® select Product # s16321). Western blot analysis (Fig. a) was performed using whole cell extracts from the MNK1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with MNK1 Monoclonal Antibody (E.611.4) (Product # MA5-14928, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MNK1

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using MNK1 Monoclonal Antibody (E.611.4) (Product # MA5-14928) was performed and a 51 kDa band corresponding to MNK1 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), Hep G2 (Lane 2), HEK 293 (Lane 3), HeLa (Lane 4), Neuro 2a (Lane 5), C2C12 (Lane 6), U2-OS (Lane 7), Mouse Lung (Lane 8) Mouse Kidney (Lane 9) and Mouse Heart (Lane 10) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence using Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).