Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-34742 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thioredoxin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: H1299, HeLaS3. Predicted reactivity: Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.69 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Thioredoxin 1 Polyclonal Antibody (Product # PA5-34742). Sample (30 µg of whole cell lysate). A: H1299. B: HeLa S3. 12% SDS PAGE. Thioredoxin 1 Polyclonal Antibody (Product # PA5-34742) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Thioredoxin 1 was achieved by transfecting U-87 MG with Thioredoxin 1 specific siRNAs (Silencer® select Product # S2, S1). Western blot analysis (Fig. a) was performed using Membrane enriched extracts from the Thioredoxin 1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Thioredoxin 1 Polyclonal Antibody (Product # PA5-34742, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Thioredoxin 1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Thioredoxin 1 Polyclonal Antibody(Product # PA5-34742) and a 11kDa band along with uncharacterized bands (*) corresponding to Thioredoxin 1 was observed across cell line tested. Membrane enriched extracts (30 µg lysate) of U-87 MG (Lane 1), MCF7 (Lane 2) and HEK-293 (Lane 3) were electrophoresed using Novex™ 16% Tricine Protein Gel (Product # EC6695BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Thioredoxin 1 was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: Thioredoxin 1 Polyclonal Antibody (Product # PA5 34742) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.