Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00007295-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007295-M01, RRID:AB_548654
- Product name
- Mouse Monoclonal Thioredoxin-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TXN - thioredoxin
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Global analysis of erythroid cells redox status reveals the involvement of Prdx1 and Prdx2 in the severity of beta thalassemia.
Crosstalk between Nrf2 and YAP contributes to maintaining the antioxidant potential and chemoresistance in bladder cancer.
Response of esophageal cancer cells to epigenetic inhibitors is mediated via altered thioredoxin activity.
Romanello KS, Teixeira KKL, Silva JPMO, Nagamatsu ST, Bezerra MAC, Domingos IF, Martins DAP, Araujo AS, Lanaro C, Breyer CA, Ferreira RA, Franco-Penteado C, Costa FF, Malavazi I, Netto LES, de Oliveira MA, Cunha AF
PloS one 2018;13(12):e0208316
PloS one 2018;13(12):e0208316
Crosstalk between Nrf2 and YAP contributes to maintaining the antioxidant potential and chemoresistance in bladder cancer.
Ciamporcero E, Daga M, Pizzimenti S, Roetto A, Dianzani C, Compagnone A, Palmieri A, Ullio C, Cangemi L, Pili R, Barrera G
Free radical biology & medicine 2018 Feb 1;115:447-457
Free radical biology & medicine 2018 Feb 1;115:447-457
Response of esophageal cancer cells to epigenetic inhibitors is mediated via altered thioredoxin activity.
Ahrens TD, Timme S, Ostendorp J, Bogatyreva L, Hoeppner J, Hopt UT, Hauschke D, Werner M, Lassmann S
Laboratory investigation; a journal of technical methods and pathology 2016 Mar;96(3):307-16
Laboratory investigation; a journal of technical methods and pathology 2016 Mar;96(3):307-16
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of TXN expression in transfected 293T cell line by TXN monoclonal antibody (M01), clone 2A7.Lane 1: TXN transfected lysate(11.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Thioredoxin Antibody (2A7) [H00007295-M01] - TXN monoclonal antibody (M01), clone 2A7 Analysis of TXN expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Thioredoxin Antibody (2A7) [H00007295-M01] - Detection limit for recombinant GST tagged TXN is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of monoclonal antibody to TXN on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of TXN transfected lysate using anti-TXN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TXN MaxPab rabbit polyclonal antibody.