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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- RQ4619 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TRX Antibody / Thioredoxin / TXN
- Antibody type
- Polyclonal
- Description
- This highly specific TRX antibody is suitable for use in Western blot/Immunohistochemistry/Immunocytochemistry/Direct ELISA/Immunofluorescence applications with mouse and rat samples.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the TRX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse 1) testis, 2) kidney, 3) small intestine, 4) spleen, 5) stomach, 6) lung, 7) smooth muscle and 8) heart lysate with TXN antibody at 0.5ug/ml. Predicted molecular weight ~12 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of rat 1) testis, 2) kidney, 3) small intestine, 4) spleen, 5) stomach, 6) lung, 7) smooth muscle and 8) heart lysate with TXN antibody at 0.5ug/ml. Predicted molecular weight ~12 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC staining of FFPE mouse LLC cells with TRX antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse small intestine with TRX antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat spleen with TRX antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
