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- Validations
- Western blot [1]
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- Product number
- PA5-57178 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RABL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LAEDFNPEEI NLDCTNPRYL AAGSSNAVKL SRFFDKVIEK RYFLREGNQI PGFPDRKRFG AGTLKSLHYD
- Concentration
- Lot-Specific
Submitted references circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression.
Ma Y, Ren Y, Wen H, Cui C
Journal of immunology research 2021;2021:6724854
Journal of immunology research 2021;2021:6724854
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RABL3 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a RABL3 Polyclonal Antibody (Product # PA5-57178).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RABL3 in human kidney tissue shows moderate nuclear membrane and cytoplasmic positivity in cells in tubules. Samples were probed using a RABL3 Polyclonal Antibody (Product # PA5-57178).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 RABL3 is the target of miR-145 and regulated by circCOL1A1. (a) StarBase V3.0 predicted that RABL3 was the target gene of miR-145. The binding site was shown as indicated. (b) Luciferase reporter assays were performed in AGS transfected with wild type and mutant miR-145. (c) Luciferase reporter assays were performed in MKN-45 transfected with wild type and mutant miR-145. (d) Relative expression of RABL3 mRNA in NC and circCOL1A1 silenced AGS cells which were transfected with miR-145 mimics or inhibitors. (e) Relative expression of RABL3 mRNA in Vector and circCOL1A1 overexpressing MKN-45 cells which were transfected with miR-145 inhibitors or mimics. (f) Protein level of RABL3 was identified by western blot in AGS cell line. (g) Protein level of RABL3 was identified by western blot in the MKN-45 cell line. (h, i) RABL3 level was identified in gastric cancer tissue.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 circCOL1A/miR-145/RABL3 axis regulates the proliferation, migration, and invasion of gastric cancer. (a) Western blot results of RABL3 in AGS cells. (b) Western blot results of RABL3 in MKN-45 cells. (c) CCK8 assay was used in AGS NC and si-circCOL1A1 cells. AGS NC and si-circCOL1A1 cells were rescued by transfection of the miR-145 inhibitor or RABL3 overexpression plasmids. (d) CCK8 assay was used in the MKN-45 vector and circCOL1A1 overexpression cells. MKN-45 vector and circCOL1A1 overexpression cells were then transfected with miR-145 mimics or RABL3 siRNA. (e) Migration assay was used in AGS NC and si-circCOL1A1 cells with indicated transfections. (f) Migration assay was used in the MKN-45 vector and circCOL1A1 overexpression cells with indicated transfections. (g) Invasion assay was used in AGS NC and si-circCOL1A1 cells with indicated transfections. (h) Invasion assay was used in the MKN-45 vector and circCOL1A1 overexpression cells with indicated transfections.
