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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 13527-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13527-1-AP, RRID:AB_2096200
- Product name
- EIF5B antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated EIF5B antibody (Cat. #13527-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins.
Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation.
eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B.
Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.
Saba JA, Huang Z, Schole KL, Ye X, Bhatt SD, Li Y, Timp W, Cheng J, Green R
bioRxiv : the preprint server for biology 2023 Nov 2;
bioRxiv : the preprint server for biology 2023 Nov 2;
Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation.
Mao Y, Jia L, Dong L, Shu XE, Qian SB
bioRxiv : the preprint server for biology 2023 Feb 16;
bioRxiv : the preprint server for biology 2023 Feb 16;
eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B.
Kim E, Kim JH, Seo K, Hong KY, An SWA, Kwon J, Lee SV, Jang SK
Cellular and molecular life sciences : CMLS 2018 Dec;75(23):4287-4300
Cellular and molecular life sciences : CMLS 2018 Dec;75(23):4287-4300
Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.
Jiang X, Jiang X, Feng Y, Xu R, Wang Q, Deng H
PloS one 2016;11(12):e0168387
PloS one 2016;11(12):e0168387
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse brain tissue were subjected to SDS PAGE followed by western blot with 13527-1-AP(EIF5B antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody 13527-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13527-1-AP(EIF5B antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13527-1-AP(EIF5B antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
