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- Antigen structure
- References [10]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 13527-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13527-1-AP, RRID:AB_2096200
- Product name
- EIF5B antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated EIF5B antibody (Cat. #13527-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Cuproptosis and Disulfidptosis Converge to Empower PD-L1 Checkpoint Therapy via Cadict-Induced PD-L1 Translation.
Tumor-derived exosomal miR-199b-5p promotes proliferation and epithelial-mesenchymal transition in non-small cell lung cancer by targeting CCNL1.
USP13-Mediated selective autophagy of eIF5B promotes pathological cardiac hypertrophy.
VP3 protein of Senecavirus A promotes viral IRES-driven translation and attenuates innate immunity by specifically relocalizing hnRNPA2B1.
Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses.
LARP1 binds ribosomes and TOP mRNAs in repressed complexes.
LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins.
Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation.
eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B.
Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.
Huang S, Song S, Zhang X, Gao J, Pu R, Dai J, Wu X, Chen L, Li Q, Liu X, Zhou Q, Song M, He W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 May;13(25):e15367
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 May;13(25):e15367
Tumor-derived exosomal miR-199b-5p promotes proliferation and epithelial-mesenchymal transition in non-small cell lung cancer by targeting CCNL1.
Liu B, Rui Y, Li M, Huang L
Translational oncology 2025 Dec;62:102564
Translational oncology 2025 Dec;62:102564
USP13-Mediated selective autophagy of eIF5B promotes pathological cardiac hypertrophy.
Ye Y, Xu J, Jin C, Li X, Wu X, Zhou C, Gao L, Zhong Z, Li X, Chen S, Wei Y, Peng S, Liu S
Cellular and molecular life sciences : CMLS 2025 Dec 16;83(1):36
Cellular and molecular life sciences : CMLS 2025 Dec 16;83(1):36
VP3 protein of Senecavirus A promotes viral IRES-driven translation and attenuates innate immunity by specifically relocalizing hnRNPA2B1.
Li L, Li X, Zhong H, Li M, Wan B, He W, Zhang Y, Du Y, Chen D, Zhang W, Ji P, Jiang D, Han S
Journal of virology 2024 Sep 17;98(9):e0122724
Journal of virology 2024 Sep 17;98(9):e0122724
Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses.
Shirasaki T, Lenarcic E, Misumi I, Xie L, Fusco WG, Yonish B, Das A, Kim H, Cameron CE, Léger-Abraham M, Chen X, Cullen JM, Whitmire JK, Li Y, Duncan JA, Moorman NJ, Lemon SM
Science advances 2024 Nov 22;10(47):eadq6342
Science advances 2024 Nov 22;10(47):eadq6342
LARP1 binds ribosomes and TOP mRNAs in repressed complexes.
Saba JA, Huang Z, Schole KL, Ye X, Bhatt SD, Li Y, Timp W, Cheng J, Green R
The EMBO journal 2024 Dec;43(24):6555-6572
The EMBO journal 2024 Dec;43(24):6555-6572
LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins.
Saba JA, Huang Z, Schole KL, Ye X, Bhatt SD, Li Y, Timp W, Cheng J, Green R
bioRxiv : the preprint server for biology 2023 Nov 2;
bioRxiv : the preprint server for biology 2023 Nov 2;
Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation.
Mao Y, Jia L, Dong L, Shu XE, Qian SB
bioRxiv : the preprint server for biology 2023 Feb 16;
bioRxiv : the preprint server for biology 2023 Feb 16;
eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B.
Kim E, Kim JH, Seo K, Hong KY, An SWA, Kwon J, Lee SV, Jang SK
Cellular and molecular life sciences : CMLS 2018 Dec;75(23):4287-4300
Cellular and molecular life sciences : CMLS 2018 Dec;75(23):4287-4300
Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.
Jiang X, Jiang X, Feng Y, Xu R, Wang Q, Deng H
PloS one 2016;11(12):e0168387
PloS one 2016;11(12):e0168387
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse brain tissue were subjected to SDS PAGE followed by western blot with 13527-1-AP(EIF5B antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody 13527-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13527-1-AP(EIF5B antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13527-1-AP(EIF5B antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
