A301-745A

antibody from Invitrogen Antibodies

Targeting: EIF5B DKFZp434I036, FLJ10524, IF2, KIAA0741

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A301-745A

Provider

Invitrogen Antibodies

Product name

eIF5B/IF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human eIF5B/IF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF5B/IF2 antibody (Product # A301-745A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. eIF5B/IF2 was immunoprecipitated successfully when using rabbit anti-eIF5B/IF2 antibody (Product # A301-744A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human eIF5B/IF2 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF5B/IF2 antibody A301-745A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. eIF5B/IF2 was immunoprecipitated successfully when using rabbit anti-eIF5B/IF2 antibody A301-744A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).