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- Antibody Data
- Antigen structure
- References [44]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- 66200-1-Ig - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#66200-1-Ig, RRID:AB_2722562
- Product name
- acetylated Tubulin(Lys40) antibody
- Antibody type
- Monoclonal
- Description
- acetylated Tubulin(Lys40) antibody (Cat. #66200-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat, Canine, pig and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat, Canine, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 7E5H8
- Vial size
- 20ul, 150ul
Submitted references Biallelic mutations in spermatogenesis and centriole-associated 1 like (SPATC1L) cause acephalic spermatozoa syndrome and male infertility.
Analysis of Key Distinct Biological Characteristics of Human Placenta-Derived Mesenchymal Stromal Cells and Individual Heterogeneity Attributing to Donors.
The histone deacetylase inhibitor PCI-24781 impairs calcium influx and inhibits proliferation and metastasis in breast cancer.
Biallelic mutations in KATNAL2 cause male infertility due to oligo-astheno-teratozoospermia.
Biallelic mutations of CFAP58 are associated with multiple morphological abnormalities of the sperm flagella.
Mutations in DNAH8 contribute to multiple morphological abnormalities of sperm flagella and male infertility.
Sub-toxic levels of cobalt ions impair chondrocyte mechanostranduction via HDAC6-dependent primary cilia shortening.
UTX/KDM6A deletion promotes the recovery of spinal cord injury by epigenetically triggering intrinsic neural regeneration.
SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.
Nasal ciliated cells are primary targets for SARS-CoV-2 replication in the early stage of COVID-19.
Phosphosite T674A mutation in kinesin family member 3A fails to reproduce tissue and ciliary defects characteristic of CILK1 loss of function.
HDAC6 Regulates the Fusion of Autophagosome and Lysosome to Involve in Odontoblast Differentiation.
UNG2 deacetylation confers cancer cell resistance to hydrogen peroxide-induced cytotoxicity.
MiR-107 confers chemoresistance to colorectal cancer by targeting calcium-binding protein 39.
Photo-triggered self-destructive ROS-responsive nanoparticles of high paclitaxel/chlorin e6 co-loading capacity for synergetic chemo-photodynamic therapy.
Loss-of-function mutations in centrosomal protein 112 is associated with human acephalic spermatozoa phenotype.
HDAC11 promotes meiotic apparatus assembly during mouse oocyte maturation via decreasing H4K16 and α-tubulin acetylation.
EBV-miR-BART12 accelerates migration and invasion in EBV-associated cancer cells by targeting tubulin polymerization-promoting protein 1.
Improved early development potence of in vitro fertilization embryos by treatment with tubacin increasing acetylated tubulin of matured porcine oocytes.
Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss.
Sirtuin 2 Inhibition Attenuates Sevoflurane-Induced Learning and Memory Deficits in Developing Rats via Modulating Microglial Activation.
Alternatively Polarized Macrophages Regulate the Growth and Differentiation of Ependymal Stem Cells through the SIRT2 Pathway.
Novel IFT140 variants cause spermatogenic dysfunction in humans.
Interaction of hnRNP K with MAP 1B-LC1 promotes TGF-β1-mediated epithelial to mesenchymal transition in lung cancer cells.
A virus-induced kidney disease model based on organ-on-a-chip: Pathogenesis exploration of virus-related renal dysfunctions.
Biallelic mutations in Sperm flagellum 2 cause human multiple morphological abnormalities of the sperm flagella (MMAF) phenotype.
The selective HDAC6 inhibitor Nexturastat A induces apoptosis, overcomes drug resistance and inhibits tumor growth in multiple myeloma.
SIRT2 Inhibition Results in Meiotic Arrest, Mitochondrial Dysfunction, and Disturbance of Redox Homeostasis during Bovine Oocyte Maturation.
Novel Mutations in CFAP44 and CFAP43 Cause Multiple Morphological Abnormalities of the Sperm Flagella (MMAF).
Biallelic mutations in PMFBP1 cause acephalic spermatozoa.
KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.
CCDC9 is identified as a novel candidate gene of severe asthenozoospermia.
Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.
EIF4G1 is a novel candidate gene associated with severe asthenozoospermia.
Stabilized Peptide HDAC Inhibitors Derived from HDAC1 Substrate H3K56 for the Treatment of Cancer Stem-Like Cells In Vivo.
Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury.
C11orf70 Mutations Disrupting the Intraflagellar Transport-Dependent Assembly of Multiple Axonemal Dyneins Cause Primary Ciliary Dyskinesia.
Spermine increases acetylation of tubulins and facilitates autophagic degradation of prion aggregates.
Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.
HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.
RPGR, a prenylated retinal ciliopathy protein, is targeted to cilia in a prenylation- and PDE6D-dependent manner.
ProBDNF inhibits collective migration and chemotaxis of rat Schwann cells.
NDUFV2 regulates neuronal migration in the developing cerebral cortex through modulation of the multipolar-bipolar transition.
The oncoprotein HBXIP promotes migration of breast cancer cells via GCN5-mediated microtubule acetylation.
Li YZ, Li N, Liu WS, Sha YW, Wu RF, Tang YL, Zhu XS, Wei XL, Zhang XY, Wang YF, Lu ZX, Zhang FX
Asian journal of andrology 2022 Jan-Feb;24(1):67-72
Asian journal of andrology 2022 Jan-Feb;24(1):67-72
Analysis of Key Distinct Biological Characteristics of Human Placenta-Derived Mesenchymal Stromal Cells and Individual Heterogeneity Attributing to Donors.
Tai C, Wang L, Xie Y, Gao T, Huang F, Wang B
Cells, tissues, organs 2021;210(1):45-57
Cells, tissues, organs 2021;210(1):45-57
The histone deacetylase inhibitor PCI-24781 impairs calcium influx and inhibits proliferation and metastasis in breast cancer.
Yang T, Wang P, Yin X, Zhang J, Huo M, Gao J, Li G, Teng X, Yu H, Huang W, Wang Y
Theranostics 2021;11(5):2058-2076
Theranostics 2021;11(5):2058-2076
Biallelic mutations in KATNAL2 cause male infertility due to oligo-astheno-teratozoospermia.
Wei X, Liu W, Zhu X, Li Y, Zhang X, Chen J, Isachenko V, Sha Y, Lu Z
Clinical genetics 2021 Oct;100(4):376-385
Clinical genetics 2021 Oct;100(4):376-385
Biallelic mutations of CFAP58 are associated with multiple morphological abnormalities of the sperm flagella.
Sha Y, Sha Y, Liu W, Zhu X, Weng M, Zhang X, Wang Y, Zhou H
Clinical genetics 2021 Mar;99(3):443-448
Clinical genetics 2021 Mar;99(3):443-448
Mutations in DNAH8 contribute to multiple morphological abnormalities of sperm flagella and male infertility.
Weng M, Sha Y, Zeng YU, Huang N, Liu W, Zhang X, Zhou H
Acta biochimica et biophysica Sinica 2021 Mar 26;53(4):472-480
Acta biochimica et biophysica Sinica 2021 Mar 26;53(4):472-480
Sub-toxic levels of cobalt ions impair chondrocyte mechanostranduction via HDAC6-dependent primary cilia shortening.
Wu H, Wang Z, Liu S, Meng H, Liu S, Fu S
Biochemical and biophysical research communications 2021 Mar 12;544:38-43
Biochemical and biophysical research communications 2021 Mar 12;544:38-43
UTX/KDM6A deletion promotes the recovery of spinal cord injury by epigenetically triggering intrinsic neural regeneration.
Guo Z, Li C, Cao Y, Qin T, Jiang L, Xu Y, Li M, Luo Z, Hu J, Lu H
Molecular therapy. Methods & clinical development 2021 Mar 12;20:337-349
Molecular therapy. Methods & clinical development 2021 Mar 12;20:337-349
SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.
Zhao Y, Xie L, Shen C, Qi Q, Qin Y, Xing J, Zhou D, Qi Y, Yan Z, Lin X, Dai R, Lin J, Yu W
Aging cell 2021 Jun;20(6):e13391
Aging cell 2021 Jun;20(6):e13391
Nasal ciliated cells are primary targets for SARS-CoV-2 replication in the early stage of COVID-19.
Ahn JH, Kim J, Hong SP, Choi SY, Yang MJ, Ju YS, Kim YT, Kim HM, Rahman MDT, Chung MK, Hong SD, Bae H, Lee CS, Koh GY
The Journal of clinical investigation 2021 Jul 1;131(13)
The Journal of clinical investigation 2021 Jul 1;131(13)
Phosphosite T674A mutation in kinesin family member 3A fails to reproduce tissue and ciliary defects characteristic of CILK1 loss of function.
Gailey CD, Wang EJ, Jin L, Ahmadi S, Brautigan DL, Li X, Xu W, Scott MM, Fu Z
Developmental dynamics : an official publication of the American Association of Anatomists 2021 Feb;250(2):263-273
Developmental dynamics : an official publication of the American Association of Anatomists 2021 Feb;250(2):263-273
HDAC6 Regulates the Fusion of Autophagosome and Lysosome to Involve in Odontoblast Differentiation.
Zhan Y, Wang H, Zhang L, Pei F, Chen Z
Frontiers in cell and developmental biology 2020;8:605609
Frontiers in cell and developmental biology 2020;8:605609
UNG2 deacetylation confers cancer cell resistance to hydrogen peroxide-induced cytotoxicity.
Bao Y, Tong L, Song B, Liu G, Zhu Q, Lu X, Zhang J, Lu YF, Wen H, Tian Y, Sun Y, Zhu WG
Free radical biology & medicine 2020 Nov 20;160:403-417
Free radical biology & medicine 2020 Nov 20;160:403-417
MiR-107 confers chemoresistance to colorectal cancer by targeting calcium-binding protein 39.
Liang Y, Zhu D, Hou L, Wang Y, Huang X, Zhou C, Zhu L, Wang Y, Li L, Gu Y, Luo M, Wang J, Meng X
British journal of cancer 2020 Mar;122(5):705-714
British journal of cancer 2020 Mar;122(5):705-714
Photo-triggered self-destructive ROS-responsive nanoparticles of high paclitaxel/chlorin e6 co-loading capacity for synergetic chemo-photodynamic therapy.
Yang X, Shi X, Zhang Y, Xu J, Ji J, Ye L, Yi F, Zhai G
Journal of controlled release : official journal of the Controlled Release Society 2020 Jul 10;323:333-349
Journal of controlled release : official journal of the Controlled Release Society 2020 Jul 10;323:333-349
Loss-of-function mutations in centrosomal protein 112 is associated with human acephalic spermatozoa phenotype.
Sha Y, Wang X, Yuan J, Zhu X, Su Z, Zhang X, Xu X, Wei X
Clinical genetics 2020 Feb;97(2):321-328
Clinical genetics 2020 Feb;97(2):321-328
HDAC11 promotes meiotic apparatus assembly during mouse oocyte maturation via decreasing H4K16 and α-tubulin acetylation.
Sui L, Zhang S, Huang R, Li Z
Cell cycle (Georgetown, Tex.) 2020 Feb;19(3):354-362
Cell cycle (Georgetown, Tex.) 2020 Feb;19(3):354-362
EBV-miR-BART12 accelerates migration and invasion in EBV-associated cancer cells by targeting tubulin polymerization-promoting protein 1.
Wu Y, Wang D, Wei F, Xiong F, Zhang S, Gong Z, Shi L, Li X, Xiang B, Ma J, Deng H, He Y, Liao Q, Zhang W, Li X, Li Y, Guo C, Zeng Z, Li G, Xiong W
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Dec;34(12):16205-16223
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Dec;34(12):16205-16223
Improved early development potence of in vitro fertilization embryos by treatment with tubacin increasing acetylated tubulin of matured porcine oocytes.
Sun J, Cui K, Li ZP, Gao B, Huang B, Liu Q, Shi D
Mechanisms of development 2020 Dec;164:103631
Mechanisms of development 2020 Dec;164:103631
Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss.
Cui L, Zheng J, Zhao Q, Chen JR, Liu H, Peng G, Wu Y, Chen C, He Q, Shi H, Yin S, Friedman RA, Chen Y, Guan MX
JCI insight 2020 Dec 3;5(23)
JCI insight 2020 Dec 3;5(23)
Sirtuin 2 Inhibition Attenuates Sevoflurane-Induced Learning and Memory Deficits in Developing Rats via Modulating Microglial Activation.
Wu Z, Zhang Y, Zhang Y, Zhao P
Cellular and molecular neurobiology 2020 Apr;40(3):437-446
Cellular and molecular neurobiology 2020 Apr;40(3):437-446
Alternatively Polarized Macrophages Regulate the Growth and Differentiation of Ependymal Stem Cells through the SIRT2 Pathway.
Ma Y, Deng M, Zhao XQ, Liu M
Experimental neurobiology 2020 Apr 30;29(2):150-163
Experimental neurobiology 2020 Apr 30;29(2):150-163
Novel IFT140 variants cause spermatogenic dysfunction in humans.
Wang X, Sha YW, Wang WT, Cui YQ, Chen J, Yan W, Hou XT, Mei LB, Yu CC, Wang J
Molecular genetics & genomic medicine 2019 Sep;7(9):e920
Molecular genetics & genomic medicine 2019 Sep;7(9):e920
Interaction of hnRNP K with MAP 1B-LC1 promotes TGF-β1-mediated epithelial to mesenchymal transition in lung cancer cells.
Li L, Yan S, Zhang H, Zhang M, Huang G, Chen M
BMC cancer 2019 Sep 6;19(1):894
BMC cancer 2019 Sep 6;19(1):894
A virus-induced kidney disease model based on organ-on-a-chip: Pathogenesis exploration of virus-related renal dysfunctions.
Wang J, Wang C, Xu N, Liu ZF, Pang DW, Zhang ZL
Biomaterials 2019 Oct;219:119367
Biomaterials 2019 Oct;219:119367
Biallelic mutations in Sperm flagellum 2 cause human multiple morphological abnormalities of the sperm flagella (MMAF) phenotype.
Sha Y, Liu W, Wei X, Zhu X, Luo X, Liang L, Guo T
Clinical genetics 2019 Nov;96(5):385-393
Clinical genetics 2019 Nov;96(5):385-393
The selective HDAC6 inhibitor Nexturastat A induces apoptosis, overcomes drug resistance and inhibits tumor growth in multiple myeloma.
Sun X, Xie Y, Sun X, Yao Y, Li H, Li Z, Yao R, Xu K
Bioscience reports 2019 Mar 29;39(3)
Bioscience reports 2019 Mar 29;39(3)
SIRT2 Inhibition Results in Meiotic Arrest, Mitochondrial Dysfunction, and Disturbance of Redox Homeostasis during Bovine Oocyte Maturation.
Xu D, Wu L, Jiang X, Yang L, Cheng J, Chen H, Hua R, Geng G, Yang L, Li Q
International journal of molecular sciences 2019 Mar 18;20(6)
International journal of molecular sciences 2019 Mar 18;20(6)
Novel Mutations in CFAP44 and CFAP43 Cause Multiple Morphological Abnormalities of the Sperm Flagella (MMAF).
Sha YW, Wang X, Xu X, Su ZY, Cui Y, Mei LB, Huang XJ, Chen J, He XM, Ji ZY, Bao H, Yang X, Li P, Li L
Reproductive sciences (Thousand Oaks, Calif.) 2019 Jan;26(1):26-34
Reproductive sciences (Thousand Oaks, Calif.) 2019 Jan;26(1):26-34
Biallelic mutations in PMFBP1 cause acephalic spermatozoa.
Sha YW, Wang X, Xu X, Ding L, Liu WS, Li P, Su ZY, Chen J, Mei LB, Zheng LK, Wang HL, Kong SB, You M, Wu JF
Clinical genetics 2019 Feb;95(2):277-286
Clinical genetics 2019 Feb;95(2):277-286
KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.
Zhao L, Zhang D, Shen Q, Jin M, Lin Z, Ma H, Huang S, Zhou P, Wu G, Zhang T
Oncogene 2019 Feb;38(7):935-949
Oncogene 2019 Feb;38(7):935-949
CCDC9 is identified as a novel candidate gene of severe asthenozoospermia.
Sha Y, Xu Y, Wei X, Liu W, Mei L, Lin S, Ji Z, Wang X, Su Z, Qiu P, Chen J, Wang X
Systems biology in reproductive medicine 2019 Dec;65(6):465-473
Systems biology in reproductive medicine 2019 Dec;65(6):465-473
Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.
Hayes CS, Labuzan SA, Menke JA, Haddock AN, Waddell DS
Journal of cellular physiology 2019 Dec;234(12):23807-23824
Journal of cellular physiology 2019 Dec;234(12):23807-23824
EIF4G1 is a novel candidate gene associated with severe asthenozoospermia.
Sha Y, Liu W, Huang X, Li Y, Ji Z, Mei L, Lin S, Kong S, Lu J, Kong L, Zhu X, Lu Z, Ding L
Molecular genetics & genomic medicine 2019 Aug;7(8):e807
Molecular genetics & genomic medicine 2019 Aug;7(8):e807
Stabilized Peptide HDAC Inhibitors Derived from HDAC1 Substrate H3K56 for the Treatment of Cancer Stem-Like Cells In Vivo.
Wang D, Li W, Zhao R, Chen L, Liu N, Tian Y, Zhao H, Xie M, Lu F, Fang Q, Liang W, Yin F, Li Z
Cancer research 2019 Apr 15;79(8):1769-1783
Cancer research 2019 Apr 15;79(8):1769-1783
Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury.
Tata A, Kobayashi Y, Chow RD, Tran J, Desai A, Massri AJ, McCord TJ, Gunn MD, Tata PR
Cell stem cell 2018 May 3;22(5):668-683.e6
Cell stem cell 2018 May 3;22(5):668-683.e6
C11orf70 Mutations Disrupting the Intraflagellar Transport-Dependent Assembly of Multiple Axonemal Dyneins Cause Primary Ciliary Dyskinesia.
Fassad MR, Shoemark A, le Borgne P, Koll F, Patel M, Dixon M, Hayward J, Richardson C, Frost E, Jenkins L, Cullup T, Chung EMK, Lemullois M, Aubusson-Fleury A, Hogg C, Mitchell DR, Tassin AM, Mitchison HM
American journal of human genetics 2018 May 3;102(5):956-972
American journal of human genetics 2018 May 3;102(5):956-972
Spermine increases acetylation of tubulins and facilitates autophagic degradation of prion aggregates.
Phadwal K, Kurian D, Salamat MKF, MacRae VE, Diack AB, Manson JC
Scientific reports 2018 Jul 3;8(1):10004
Scientific reports 2018 Jul 3;8(1):10004
Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.
Zhang DL, Sun YJ, Ma ML, Wang YJ, Lin H, Li RR, Liang ZL, Gao Y, Yang Z, He DF, Lin A, Mo H, Lu YJ, Li MJ, Kong W, Chung KY, Yi F, Li JY, Qin YY, Li J, Thomsen ARB, Kahsai AW, Chen ZJ, Xu ZG, Liu M, Li D, Yu X, Sun JP
eLife 2018 Feb 2;7
eLife 2018 Feb 2;7
HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.
Yan Y, Wang H, Hu M, Jiang L, Wang Y, Liu P, Liang X, Liu J, Li C, Lindström-Battle A, Lam SM, Shui G, Deng WM, Jiao R
Developmental cell 2017 Oct 9;43(1):99-111.e5
Developmental cell 2017 Oct 9;43(1):99-111.e5
RPGR, a prenylated retinal ciliopathy protein, is targeted to cilia in a prenylation- and PDE6D-dependent manner.
Dutta N, Seo S
Biology open 2016 Sep 15;5(9):1283-9
Biology open 2016 Sep 15;5(9):1283-9
ProBDNF inhibits collective migration and chemotaxis of rat Schwann cells.
Ding YQ, Li XY, Xia GN, Ren HY, Zhou XF, Su BY, Qi JG
Tissue & cell 2016 Oct;48(5):503-10
Tissue & cell 2016 Oct;48(5):503-10
NDUFV2 regulates neuronal migration in the developing cerebral cortex through modulation of the multipolar-bipolar transition.
Chen T, Wu Q, Zhang Y, Zhang D
Brain research 2015 Nov 2;1625:102-10
Brain research 2015 Nov 2;1625:102-10
The oncoprotein HBXIP promotes migration of breast cancer cells via GCN5-mediated microtubule acetylation.
Li L, Liu B, Zhang X, Ye L
Biochemical and biophysical research communications 2015 Mar 13;458(3):720-725
Biochemical and biophysical research communications 2015 Mar 13;458(3):720-725
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 66200-1-Ig( TUBA1A(ace-40Lys) Antibody) at dilution of 1:1000
- Other comments
- WB
Supportive validation
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The Acetylated tubulin (Lys40) antibody from Proteintech is a mouse monoclonal antibody to a peptide of human Acetylated tubulin (Lys40). This antibody recognizes human, mouse, rat antigen. The Acetylated tubulin (Lys40) antibody has been validated for the following applications: ELISA, IF, WB analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MDCK cells using 66200-1-Ig( TUBA1A(ace-40Lys) Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
- Other comments
- IF
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 66200-1-Ig( TUBA1A(ace-40Lys) Antibody) at dilution of 1:50 (under 40x lens)
- Other comments
- IHC
