NB100-690
antibody from Novus Biologicals
Targeting: TUBA1A
B-ALPHA-1, FLJ25113, TUBA3
Antibody data
- Antibody Data
- Antigen structure
- References [84]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [9]
- Flow cytometry [6]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-690 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-690, RRID:AB_2210209
- Product name
- Mouse Monoclonal alpha Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This alpha Tubulin Antibody (DM1A) does not cross-react with beta Tubulin.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Goat, Guinea Pig, Hamster, Porcine, Rabbit, Simian, Xenopus, Yeast
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Safety, Tumor Reduction, and Clinical Impact of Zika Virus Injection in Dogs with Advanced-Stage Brain Tumors.
C-C motif chemokine receptor 2 as a novel intermediate in the ovulatory cascade.
Engineering Chimeric Antigen Receptor T Cells against Immune Checkpoint Inhibitors PD-1/PD-L1 for Treating Pancreatic Cancer.
Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness.
TRPM7 contributes to progressive nephropathy.
Neurofilaments form flexible bundles during neuritogenesis in culture and in mature axons in situ.
Combined Omics Reveals That Disruption of the Selenocysteine Lyase Gene Affects Amino Acid Pathways in Mice.
Identification of Novel Adenylyl Cyclase 5 (AC5) Signaling Networks in D1 and D2 Medium Spiny Neurons using Bimolecular Fluorescence Complementation Screening.
Functional characterization of AC5 gain-of-function variants: Impact on the molecular basis of ADCY5-related dyskinesia.
SETD1A protects from senescence through regulation of the mitotic gene expression program.
Mammalian orthoreovirus Infection is Enhanced in Cells Pre-Treated with Sodium Arsenite.
Guggulsterone Activates Adipocyte Beiging through Direct Effects on 3T3-L1 Adipocytes and Indirect Effects Mediated through RAW264.7 Macrophages.
Therapeutic effects of statins against lung adenocarcinoma via p53 mutant-mediated apoptosis.
Induction of the Antioxidant Response by the Transcription Factor NRF2 Increases Bioactivation of the Mutagenic Air Pollutant 3-Nitrobenzanthrone in Human Lung Cells.
Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines.
Serum amyloid A1 in combination with integrin αVβ3 increases glioblastoma cells mobility and progression.
N-glycosylation of the AMPA-type glutamate receptor regulates cell surface expression and tetramer formation affecting channel function.
A tripartite mode of action approach for investigating the impact of aneugens on tubulin polymerization.
Selenoprotein S Reduces Endoplasmic Reticulum Stress-Induced Phosphorylation of Tau: Potential Role in Selenate Mitigation of Tau Pathology.
UBX domain-containing proteins are involved in lipid homeostasis and stress responses in Pichia pastoris.
Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis.
Overexpression of Mitogen-activated protein kinase phosphatase-3 (MKP-3) reduces FoxO1 phosphorylation in mice hypothalamus.
Cytoskeleton-related regulation of primary cilia shortening mediated by melanin-concentrating hormone receptor 1.
Decitabine inhibits tumor cell proliferation and up-regulates e-cadherin expression in Epstein-Barr virus-associated gastric cancer.
Opposing Effects of Valproic Acid Treatment Mediated by Histone Deacetylase Inhibitor Activity in Four Transgenic X. laevis Models of Retinitis Pigmentosa.
Statins Increase Mitochondrial and Peroxisomal Fatty Acid Oxidation in the Liver and Prevent Non-Alcoholic Steatohepatitis in Mice.
Ablation of Perk in Schwann Cells Improves Myelination in the S63del Charcot-Marie-Tooth 1B Mouse.
The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer.
Oncogenic roles of carbonic anhydrase 8 in human osteosarcoma cells.
N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability.
The role of endogenous opioid peptides in the antinociceptive effect of 15-deoxy(Δ12,14)-prostaglandin J2 in the temporomandibular joint.
Low Level Pro-inflammatory Cytokines Decrease Connexin36 Gap Junction Coupling in Mouse and Human Islets through Nitric Oxide-mediated Protein Kinase Cδ.
Hinokitiol inhibits vasculogenic mimicry activity of breast cancer stem/progenitor cells through proteasome-mediated degradation of epidermal growth factor receptor.
Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells.
Synergistic anticancer potential of dichloroacetate and estradiol analogue exerting their effect via ROS-JNK-Bcl-2-mediated signalling pathways.
Retinoic acid-induced IgG production in TLR-activated human primary B cells involves ULK1-mediated autophagy.
Using cytochrome P4501A1 expression in liver and blubber to understand effects of persistent organic pollutant exposure in stranded Pacific Island cetaceans.
Convergent Regulation of Candida albicans Aft2 and Czf1 in Invasive and Opaque Filamentation.
Diet-induced obesity in the selenocysteine lyase knockout mouse.
Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease.
Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics.
Human SLC4A11 Is a Novel NH3/H+ Co-transporter.
Identification of heme oxygenase-1 stimulators by a convenient ELISA-based bilirubin quantification assay.
Candida albicans autophagy, no longer a bystander: Its role in tolerance to ER stress-related antifungal drugs.
Double-Edge Sword of Sustained ROCK Activation in Prion Diseases through Neuritogenesis Defects and Prion Accumulation.
SapC-DOPS nanovesicles induce Smac- and Bax-dependent apoptosis through mitochondrial activation in neuroblastomas.
Neuroprotective role of sphingosine-1-phosphate in L-BMAA treated neuroblastoma cells (SH-SY5Y).
Immunolocalization of antimicrobial and cytoskeletal components in the serous glands of human sinonasal mucosa.
Fatty acid and phospholipid biosynthetic pathways are regulated throughout mammary epithelial cell differentiation and correlate to breast cancer survival.
Autophagy is required for G₁/G₀ quiescence in response to nitrogen starvation in Saccharomyces cerevisiae.
Interaction among the vacuole, the mitochondria, and the oxidative stress response is governed by the transient receptor potential channel in Candida albicans.
Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype.
Endothelin-1 induces connective tissue growth factor expression in human lung fibroblasts by ETAR-dependent JNK/AP-1 pathway.
Vascular disrupting effect of CKD-516: preclinical study using DCE-MRI.
IMP1 promotes choriocarcinoma cell migration and invasion through the novel effectors RSK2 and PPME1.
Novel insight into the expression and function of the multicopper oxidases in Candida albicans.
Gene expression deficits in pontine locus coeruleus astrocytes in men with major depressive disorder.
Methamphetamine decreases levels of glutathione peroxidases 1 and 4 in SH-SY5Y neuronal cells: protective effects of selenium.
Reduced proficiency in homologous recombination underlies the high sensitivity of embryonal carcinoma testicular germ cell tumors to Cisplatin and poly (adp-ribose) polymerase inhibition.
Disruption of the selenocysteine lyase-mediated selenium recycling pathway leads to metabolic syndrome in mice.
Herpes simplex virus γ34.5 interferes with autophagosome maturation and antigen presentation in dendritic cells.
Hydrodynamic stretching of single cells for large population mechanical phenotyping.
Impact of partial urethral obstruction on bladder function: time-dependent changes and functional correlates of altered expression of Ca²⁺ signaling regulators.
Absence of selenoprotein P but not selenocysteine lyase results in severe neurological dysfunction.
Thrombin induced connective tissue growth factor expression in rat vascular smooth muscle cells via the PAR-1/JNK/AP-1 pathway.
TEX11 modulates germ cell proliferation by competing with estrogen receptor β for the binding to HPIP.
Selenoprotein K is a novel target of m-calpain, and cleavage is regulated by Toll-like receptor-induced calpastatin in macrophages.
p53 in trichostatin A induced C6 glioma cell death.
Targeting RAD51 phosphotyrosine-315 to prevent unfaithful recombination repair in BCR-ABL1 leukemia.
Selenoprotein K knockout mice exhibit deficient calcium flux in immune cells and impaired immune responses.
Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection.
MAPK phosphatase-1 contributes to trichostatin A inhibition of cyclooxygenase-2 expression in human umbilical vascular endothelial cells exposed to lipopolysaccharide.
Specific antioxidant selenoproteins are induced in the heart during hypertrophy.
Cell-cell junctions and vascular endothelial growth factor in rat lung as affected by ischemia/reperfusion and preconditioning with inhaled nitric oxide.
Glycogen synthase kinase-3/Shaggy mediates ethanol-induced excitotoxic cell death of Drosophila olfactory neurons.
Proteomic studies on low- and high-grade human brain astrocytomas.
G protein binding and G protein activation by nucleotide transfer involve distinct domains on tubulin: regulation of signal transduction by cytoskeletal elements.
G protein binding and G protein activation by nucleotide transfer involve distinct domains on tubulin: regulation of signal transduction by cytoskeletal elements.
The role of microtubules in platelet secretory release.
The role of microtubules in platelet secretory release.
Carboxy-terminal regions on the surface of tubulin and microtubules. Epitope locations of YOL1/34, DM1A and DM1B.
Carboxy-terminal regions on the surface of tubulin and microtubules. Epitope locations of YOL1/34, DM1A and DM1B.
10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin.
10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin.
Kaid C, Madi RADS, Astray R, Goulart E, Caires-Junior LC, Mitsugi TG, Moreno ACR, Castro-Amarante MF, Pereira LR, Porchia BFMM, de Andrade TO, Landini V, Sanches DS, Pires CG, Tanioka RKO, Pereira MCL, Barbosa IN, Massoco CO, Ferreira LCS, Okamoto OK, Zatz M
Molecular therapy : the journal of the American Society of Gene Therapy 2020 May 6;28(5):1276-1286
Molecular therapy : the journal of the American Society of Gene Therapy 2020 May 6;28(5):1276-1286
C-C motif chemokine receptor 2 as a novel intermediate in the ovulatory cascade.
Jaworski JP, Urrutia M, Dascal E, Jaita G, Peluffo MC
Molecular human reproduction 2020 May 15;26(5):289-300
Molecular human reproduction 2020 May 15;26(5):289-300
Engineering Chimeric Antigen Receptor T Cells against Immune Checkpoint Inhibitors PD-1/PD-L1 for Treating Pancreatic Cancer.
Yang CY, Fan MH, Miao CH, Liao YJ, Yuan RH, Liu CL
Molecular therapy oncolytics 2020 Jun 26;17:571-585
Molecular therapy oncolytics 2020 Jun 26;17:571-585
Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness.
Lu H, Xie Y, Tran L, Lan J, Yang Y, Murugan NL, Wang R, Wang YJ, Semenza GL
The Journal of clinical investigation 2020 Jul 27;
The Journal of clinical investigation 2020 Jul 27;
TRPM7 contributes to progressive nephropathy.
Suzuki S, Penner R, Fleig A
Scientific reports 2020 Feb 11;10(1):2333
Scientific reports 2020 Feb 11;10(1):2333
Neurofilaments form flexible bundles during neuritogenesis in culture and in mature axons in situ.
Lee S, Eyer J, Letournel F, Boumil E, Hall G, Shea TB
Journal of neuroscience research 2019 Oct;97(10):1306-1318
Journal of neuroscience research 2019 Oct;97(10):1306-1318
Combined Omics Reveals That Disruption of the Selenocysteine Lyase Gene Affects Amino Acid Pathways in Mice.
Seale LA, Khadka VS, Menor M, Xie G, Watanabe LM, Sasuclark A, Guirguis K, Ha HY, Hashimoto AC, Peplowska K, Tiirikainen M, Jia W, Berry MJ, Deng Y
Nutrients 2019 Oct 26;11(11)
Nutrients 2019 Oct 26;11(11)
Identification of Novel Adenylyl Cyclase 5 (AC5) Signaling Networks in D1 and D2 Medium Spiny Neurons using Bimolecular Fluorescence Complementation Screening.
Doyle TB, Muntean BS, Ejendal KF, Hayes MP, Soto-Velasquez M, Martemyanov KA, Dessauer CW, Hu CD, Watts VJ
Cells 2019 Nov 19;8(11)
Cells 2019 Nov 19;8(11)
Functional characterization of AC5 gain-of-function variants: Impact on the molecular basis of ADCY5-related dyskinesia.
Doyle TB, Hayes MP, Chen DH, Raskind WH, Watts VJ
Biochemical pharmacology 2019 May;163:169-177
Biochemical pharmacology 2019 May;163:169-177
SETD1A protects from senescence through regulation of the mitotic gene expression program.
Tajima K, Matsuda S, Yae T, Drapkin BJ, Morris R, Boukhali M, Niederhoffer K, Comaills V, Dubash T, Nieman L, Guo H, Magnus NKC, Dyson N, Shioda T, Haas W, Haber DA, Maheswaran S
Nature communications 2019 Jun 28;10(1):2854
Nature communications 2019 Jun 28;10(1):2854
Mammalian orthoreovirus Infection is Enhanced in Cells Pre-Treated with Sodium Arsenite.
Lutz MM 4th, Worth MP, Hinchman MM, Parker JSL, Ledgerwood ED
Viruses 2019 Jun 18;11(6)
Viruses 2019 Jun 18;11(6)
Guggulsterone Activates Adipocyte Beiging through Direct Effects on 3T3-L1 Adipocytes and Indirect Effects Mediated through RAW264.7 Macrophages.
Miller CN, Samuels JS, Azhar Y, Parmar A, Shashidharamurthy R, Rayalam S
Medicines (Basel, Switzerland) 2019 Jan 31;6(1)
Medicines (Basel, Switzerland) 2019 Jan 31;6(1)
Therapeutic effects of statins against lung adenocarcinoma via p53 mutant-mediated apoptosis.
Chou CW, Lin CH, Hsiao TH, Lo CC, Hsieh CY, Huang CC, Sher YP
Scientific reports 2019 Dec 31;9(1):20403
Scientific reports 2019 Dec 31;9(1):20403
Induction of the Antioxidant Response by the Transcription Factor NRF2 Increases Bioactivation of the Mutagenic Air Pollutant 3-Nitrobenzanthrone in Human Lung Cells.
Murray JR, de la Vega L, Hayes JD, Duan L, Penning TM
Chemical research in toxicology 2019 Dec 16;32(12):2538-2551
Chemical research in toxicology 2019 Dec 16;32(12):2538-2551
Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines.
Nel M, Joubert AM, Dohle W, Potter BV, Theron AE
Drug design, development and therapy 2018;12:1881-1904
Drug design, development and therapy 2018;12:1881-1904
Serum amyloid A1 in combination with integrin αVβ3 increases glioblastoma cells mobility and progression.
Lin CY, Yang ST, Shen SC, Hsieh YC, Hsu FT, Chen CY, Chiang YH, Chuang JY, Chen KY, Hsu TI, Leong WC, Su YK, Lo WL, Yeh YS, Patria YN, Shih HM, Chang CC, Chou SY
Molecular oncology 2018 May;12(5):756-771
Molecular oncology 2018 May;12(5):756-771
N-glycosylation of the AMPA-type glutamate receptor regulates cell surface expression and tetramer formation affecting channel function.
Kandel MB, Yamamoto S, Midorikawa R, Morise J, Wakazono Y, Oka S, Takamiya K
Journal of neurochemistry 2018 Dec;147(6):730-747
Journal of neurochemistry 2018 Dec;147(6):730-747
A tripartite mode of action approach for investigating the impact of aneugens on tubulin polymerization.
Stock V, Sutter A, Raschke M, Queisser N
Environmental and molecular mutagenesis 2018 Apr;59(3):188-201
Environmental and molecular mutagenesis 2018 Apr;59(3):188-201
Selenoprotein S Reduces Endoplasmic Reticulum Stress-Induced Phosphorylation of Tau: Potential Role in Selenate Mitigation of Tau Pathology.
Rueli RH, Torres DJ, Dewing AS, Kiyohara AC, Barayuga SM, Bellinger MT, Uyehara-Lock JH, White LR, Moreira PI, Berry MJ, Perry G, Bellinger FP
Journal of Alzheimer's disease : JAD 2017;55(2):749-762
Journal of Alzheimer's disease : JAD 2017;55(2):749-762
UBX domain-containing proteins are involved in lipid homeostasis and stress responses in Pichia pastoris.
Zhang M, Yu Q, Liu Z, Liang C, Zhang B, Li M
The international journal of biochemistry & cell biology 2017 Sep;90:136-144
The international journal of biochemistry & cell biology 2017 Sep;90:136-144
Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis.
Chen PC, Hsieh MH, Kuo WS, Kao HF, Hsu CL, Wang JY
The Journal of allergy and clinical immunology 2017 Oct;140(4):1146-1149.e8
The Journal of allergy and clinical immunology 2017 Oct;140(4):1146-1149.e8
Overexpression of Mitogen-activated protein kinase phosphatase-3 (MKP-3) reduces FoxO1 phosphorylation in mice hypothalamus.
Rodrigues BA, Kuga GK, Muñoz VR, Gaspar RC, Tavares MR, Botezelli JD, da Silva ASR, Cintra DE, de Moura LP, Simabuco FM, Ropelle ER, Pauli JR
Neuroscience letters 2017 Oct 17;659:14-17
Neuroscience letters 2017 Oct 17;659:14-17
Cytoskeleton-related regulation of primary cilia shortening mediated by melanin-concentrating hormone receptor 1.
Tomoshige S, Kobayashi Y, Hosoba K, Hamamoto A, Miyamoto T, Saito Y
General and comparative endocrinology 2017 Nov 1;253:44-52
General and comparative endocrinology 2017 Nov 1;253:44-52
Decitabine inhibits tumor cell proliferation and up-regulates e-cadherin expression in Epstein-Barr virus-associated gastric cancer.
Nakamura M, Nishikawa J, Saito M, Sakai K, Sasaki S, Hashimoto S, Okamoto T, Suehiro Y, Yamasaki T, Sakaida I
Journal of medical virology 2017 Mar;89(3):508-517
Journal of medical virology 2017 Mar;89(3):508-517
Opposing Effects of Valproic Acid Treatment Mediated by Histone Deacetylase Inhibitor Activity in Four Transgenic X. laevis Models of Retinitis Pigmentosa.
Vent-Schmidt RYJ, Wen RH, Zong Z, Chiu CN, Tam BM, May CG, Moritz OL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Jan 25;37(4):1039-1054
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Jan 25;37(4):1039-1054
Statins Increase Mitochondrial and Peroxisomal Fatty Acid Oxidation in the Liver and Prevent Non-Alcoholic Steatohepatitis in Mice.
Park HS, Jang JE, Ko MS, Woo SH, Kim BJ, Kim HS, Park HS, Park IS, Koh EH, Lee KU
Diabetes & metabolism journal 2016 Oct;40(5):376-385
Diabetes & metabolism journal 2016 Oct;40(5):376-385
Ablation of Perk in Schwann Cells Improves Myelination in the S63del Charcot-Marie-Tooth 1B Mouse.
Sidoli M, Musner N, Silvestri N, Ungaro D, D'Antonio M, Cavener DR, Feltri ML, Wrabetz L
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Nov 2;36(44):11350-11361
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Nov 2;36(44):11350-11361
The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer.
Hsuan SW, Chyau CC, Hung HY, Chen JH, Chou FP
European journal of nutrition 2016 Mar;55(2):491-503
European journal of nutrition 2016 Mar;55(2):491-503
Oncogenic roles of carbonic anhydrase 8 in human osteosarcoma cells.
Wang TK, Lin YM, Lo CM, Tang CH, Teng CL, Chao WT, Wu MH, Liu CS, Hsieh M
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jun;37(6):7989-8005
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jun;37(6):7989-8005
N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability.
Chang CF, Hsu LS, Weng CY, Chen CK, Wang SY, Chou YH, Liu YY, Yuan ZX, Huang WY, Lin H, Chen YH, Tsai JN
International journal of molecular sciences 2016 Jun 14;17(6)
International journal of molecular sciences 2016 Jun 14;17(6)
The role of endogenous opioid peptides in the antinociceptive effect of 15-deoxy(Δ12,14)-prostaglandin J2 in the temporomandibular joint.
Macedo CG, Napimoga MH, Rocha-Neto LM, Abdalla HB, Clemente-Napimoga JT
Prostaglandins, leukotrienes, and essential fatty acids 2016 Jul;110:27-34
Prostaglandins, leukotrienes, and essential fatty acids 2016 Jul;110:27-34
Low Level Pro-inflammatory Cytokines Decrease Connexin36 Gap Junction Coupling in Mouse and Human Islets through Nitric Oxide-mediated Protein Kinase Cδ.
Farnsworth NL, Walter RL, Hemmati A, Westacott MJ, Benninger RK
The Journal of biological chemistry 2016 Feb 12;291(7):3184-96
The Journal of biological chemistry 2016 Feb 12;291(7):3184-96
Hinokitiol inhibits vasculogenic mimicry activity of breast cancer stem/progenitor cells through proteasome-mediated degradation of epidermal growth factor receptor.
Tu DG, Yu Y, Lee CH, Kuo YL, Lu YC, Tu CW, Chang WW
Oncology letters 2016 Apr;11(4):2934-2940
Oncology letters 2016 Apr;11(4):2934-2940
Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells.
Bercht Pfleghaar K, Taimen P, Butin-Israeli V, Shimi T, Langer-Freitag S, Markaki Y, Goldman AE, Wehnert M, Goldman RD
Nucleus (Austin, Tex.) 2015;6(1):66-76
Nucleus (Austin, Tex.) 2015;6(1):66-76
Synergistic anticancer potential of dichloroacetate and estradiol analogue exerting their effect via ROS-JNK-Bcl-2-mediated signalling pathways.
Stander XX, Stander BA, Joubert AM
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2015;35(4):1499-526
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2015;35(4):1499-526
Retinoic acid-induced IgG production in TLR-activated human primary B cells involves ULK1-mediated autophagy.
Eriksen AB, Torgersen ML, Holm KL, Abrahamsen G, Spurkland A, Moskaug JØ, Simonsen A, Blomhoff HK
Autophagy 2015;11(3):460-71
Autophagy 2015;11(3):460-71
Using cytochrome P4501A1 expression in liver and blubber to understand effects of persistent organic pollutant exposure in stranded Pacific Island cetaceans.
Bachman MJ, Foltz KM, Lynch JM, West KL, Jensen BA
Environmental toxicology and chemistry 2015 Sep;34(9):1989-95
Environmental toxicology and chemistry 2015 Sep;34(9):1989-95
Convergent Regulation of Candida albicans Aft2 and Czf1 in Invasive and Opaque Filamentation.
Xu N, Dong YJ, Yu QL, Zhang B, Zhang M, Jia C, Chen YL, Zhang B, Xing LJ, Li MC
Journal of cellular biochemistry 2015 Sep;116(9):1908-18
Journal of cellular biochemistry 2015 Sep;116(9):1908-18
Diet-induced obesity in the selenocysteine lyase knockout mouse.
Seale LA, Gilman CL, Hashimoto AC, Ogawa-Wong AN, Berry MJ
Antioxidants & redox signaling 2015 Oct 1;23(10):761-74
Antioxidants & redox signaling 2015 Oct 1;23(10):761-74
Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease.
Pitts MW, Kremer PM, Hashimoto AC, Torres DJ, Byrns CN, Williams CS, Berry MJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Nov 18;35(46):15326-38
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Nov 18;35(46):15326-38
Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics.
Park SJ, Jeong J, Park YU, Park KS, Lee H, Lee N, Kim SM, Kuroda K, Nguyen MD, Kaibuchi K, Park SK
Scientific reports 2015 Mar 3;5:8694
Scientific reports 2015 Mar 3;5:8694
Human SLC4A11 Is a Novel NH3/H+ Co-transporter.
Zhang W, Ogando DG, Bonanno JA, Obukhov AG
The Journal of biological chemistry 2015 Jul 3;290(27):16894-905
The Journal of biological chemistry 2015 Jul 3;290(27):16894-905
Identification of heme oxygenase-1 stimulators by a convenient ELISA-based bilirubin quantification assay.
Rücker H, Amslinger S
Free radical biology & medicine 2015 Jan;78:135-46
Free radical biology & medicine 2015 Jan;78:135-46
Candida albicans autophagy, no longer a bystander: Its role in tolerance to ER stress-related antifungal drugs.
Yu Q, Jia C, Dong Y, Zhang B, Xiao C, Chen Y, Wang Y, Li X, Wang L, Zhang B, Li M
Fungal genetics and biology : FG & B 2015 Aug;81:238-49
Fungal genetics and biology : FG & B 2015 Aug;81:238-49
Double-Edge Sword of Sustained ROCK Activation in Prion Diseases through Neuritogenesis Defects and Prion Accumulation.
Alleaume-Butaux A, Nicot S, Pietri M, Baudry A, Dakowski C, Tixador P, Ardila-Osorio H, Haeberlé AM, Bailly Y, Peyrin JM, Launay JM, Kellermann O, Schneider B
PLoS pathogens 2015 Aug;11(8):e1005073
PLoS pathogens 2015 Aug;11(8):e1005073
SapC-DOPS nanovesicles induce Smac- and Bax-dependent apoptosis through mitochondrial activation in neuroblastomas.
Sulaiman MK, Chu Z, Blanco VM, Vallabhapurapu SD, Franco RS, Qi X
Molecular cancer 2015 Apr 8;14:78
Molecular cancer 2015 Apr 8;14:78
Neuroprotective role of sphingosine-1-phosphate in L-BMAA treated neuroblastoma cells (SH-SY5Y).
Muñoz-Sáez E, de Munck García E, Arahuetes Portero RM, Vicente F, Ortiz-López FJ, Cantizani J, Gómez Miguel B
Neuroscience letters 2015 Apr 23;593:83-9
Neuroscience letters 2015 Apr 23;593:83-9
Immunolocalization of antimicrobial and cytoskeletal components in the serous glands of human sinonasal mucosa.
Stoeckelhuber M, Olzowy B, Ihler F, Matthias C, Scherer EQ, Babaryka G, Loeffelbein DJ, Rohleder NH, Nieberler M, Kesting MR
Histology and histopathology 2014 Oct;29(10):1315-24
Histology and histopathology 2014 Oct;29(10):1315-24
Fatty acid and phospholipid biosynthetic pathways are regulated throughout mammary epithelial cell differentiation and correlate to breast cancer survival.
Dória ML, Ribeiro AS, Wang J, Cotrim CZ, Domingues P, Williams C, Domingues MR, Helguero LA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Oct;28(10):4247-64
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Oct;28(10):4247-64
Autophagy is required for G₁/G₀ quiescence in response to nitrogen starvation in Saccharomyces cerevisiae.
An Z, Tassa A, Thomas C, Zhong R, Xiao G, Fotedar R, Tu BP, Klionsky DJ, Levine B
Autophagy 2014 Oct 1;10(10):1702-11
Autophagy 2014 Oct 1;10(10):1702-11
Interaction among the vacuole, the mitochondria, and the oxidative stress response is governed by the transient receptor potential channel in Candida albicans.
Yu Q, Zhang B, Yang B, Chen J, Wang H, Jia C, Ding X, Xu N, Dong Y, Zhang B, Xing L, Li M
Free radical biology & medicine 2014 Dec;77:152-67
Free radical biology & medicine 2014 Dec;77:152-67
Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype.
Xiang L, Gilkes DM, Hu H, Takano N, Luo W, Lu H, Bullen JW, Samanta D, Liang H, Semenza GL
Oncotarget 2014 Dec 30;5(24):12509-27
Oncotarget 2014 Dec 30;5(24):12509-27
Endothelin-1 induces connective tissue growth factor expression in human lung fibroblasts by ETAR-dependent JNK/AP-1 pathway.
Weng CM, Yu CC, Kuo ML, Chen BC, Lin CH
Biochemical pharmacology 2014 Apr 1;88(3):402-11
Biochemical pharmacology 2014 Apr 1;88(3):402-11
Vascular disrupting effect of CKD-516: preclinical study using DCE-MRI.
Kim KW, Lee JM, Jeon YS, Lee IJ, Choi Y, Park J, Kiefer B, Kim C, Han JK, Choi BI
Investigational new drugs 2013 Oct;31(5):1097-106
Investigational new drugs 2013 Oct;31(5):1097-106
IMP1 promotes choriocarcinoma cell migration and invasion through the novel effectors RSK2 and PPME1.
Hsieh YT, Chou MM, Chen HC, Tseng JJ
Gynecologic oncology 2013 Oct;131(1):182-90
Gynecologic oncology 2013 Oct;131(1):182-90
Novel insight into the expression and function of the multicopper oxidases in Candida albicans.
Cheng X, Xu N, Yu Q, Ding X, Qian K, Zhao Q, Wang Y, Zhang B, Xing L, Li M
Microbiology (Reading, England) 2013 Jun;159(Pt 6):1044-1055
Microbiology (Reading, England) 2013 Jun;159(Pt 6):1044-1055
Gene expression deficits in pontine locus coeruleus astrocytes in men with major depressive disorder.
Chandley MJ, Szebeni K, Szebeni A, Crawford J, Stockmeier CA, Turecki G, Miguel-Hidalgo JJ, Ordway GA
Journal of psychiatry & neuroscience : JPN 2013 Jul;38(4):276-84
Journal of psychiatry & neuroscience : JPN 2013 Jul;38(4):276-84
Methamphetamine decreases levels of glutathione peroxidases 1 and 4 in SH-SY5Y neuronal cells: protective effects of selenium.
Barayuga SM, Pang X, Andres MA, Panee J, Bellinger FP
Neurotoxicology 2013 Jul;37:240-6
Neurotoxicology 2013 Jul;37:240-6
Reduced proficiency in homologous recombination underlies the high sensitivity of embryonal carcinoma testicular germ cell tumors to Cisplatin and poly (adp-ribose) polymerase inhibition.
Cavallo F, Graziani G, Antinozzi C, Feldman DR, Houldsworth J, Bosl GJ, Chaganti RS, Moynahan ME, Jasin M, Barchi M
PloS one 2012;7(12):e51563
PloS one 2012;7(12):e51563
Disruption of the selenocysteine lyase-mediated selenium recycling pathway leads to metabolic syndrome in mice.
Seale LA, Hashimoto AC, Kurokawa S, Gilman CL, Seyedali A, Bellinger FP, Raman AV, Berry MJ
Molecular and cellular biology 2012 Oct;32(20):4141-54
Molecular and cellular biology 2012 Oct;32(20):4141-54
Herpes simplex virus γ34.5 interferes with autophagosome maturation and antigen presentation in dendritic cells.
Gobeil PA, Leib DA
mBio 2012 Oct 16;3(5):e00267-12
mBio 2012 Oct 16;3(5):e00267-12
Hydrodynamic stretching of single cells for large population mechanical phenotyping.
Gossett DR, Tse HT, Lee SA, Ying Y, Lindgren AG, Yang OO, Rao J, Clark AT, Di Carlo D
Proceedings of the National Academy of Sciences of the United States of America 2012 May 15;109(20):7630-5
Proceedings of the National Academy of Sciences of the United States of America 2012 May 15;109(20):7630-5
Impact of partial urethral obstruction on bladder function: time-dependent changes and functional correlates of altered expression of Ca²⁺ signaling regulators.
Burmeister D, AbouShwareb T, D'Agostino R Jr, Andersson KE, Christ GJ
American journal of physiology. Renal physiology 2012 Jun 15;302(12):F1517-28
American journal of physiology. Renal physiology 2012 Jun 15;302(12):F1517-28
Absence of selenoprotein P but not selenocysteine lyase results in severe neurological dysfunction.
Raman AV, Pitts MW, Seyedali A, Hashimoto AC, Seale LA, Bellinger FP, Berry MJ
Genes, brain, and behavior 2012 Jul;11(5):601-13
Genes, brain, and behavior 2012 Jul;11(5):601-13
Thrombin induced connective tissue growth factor expression in rat vascular smooth muscle cells via the PAR-1/JNK/AP-1 pathway.
Ko WC, Chen BC, Hsu MJ, Tsai CT, Hong CY, Lin CH
Acta pharmacologica Sinica 2012 Jan;33(1):49-56
Acta pharmacologica Sinica 2012 Jan;33(1):49-56
TEX11 modulates germ cell proliferation by competing with estrogen receptor β for the binding to HPIP.
Yu YH, Siao FP, Hsu LC, Yen PH
Molecular endocrinology (Baltimore, Md.) 2012 Apr;26(4):630-42
Molecular endocrinology (Baltimore, Md.) 2012 Apr;26(4):630-42
Selenoprotein K is a novel target of m-calpain, and cleavage is regulated by Toll-like receptor-induced calpastatin in macrophages.
Huang Z, Hoffmann FW, Norton RL, Hashimoto AC, Hoffmann PR
The Journal of biological chemistry 2011 Oct 7;286(40):34830-8
The Journal of biological chemistry 2011 Oct 7;286(40):34830-8
p53 in trichostatin A induced C6 glioma cell death.
Hsu YF, Sheu JR, Hsiao G, Lin CH, Chang TH, Chiu PT, Wang CY, Hsu MJ
Biochimica et biophysica acta 2011 May;1810(5):504-13
Biochimica et biophysica acta 2011 May;1810(5):504-13
Targeting RAD51 phosphotyrosine-315 to prevent unfaithful recombination repair in BCR-ABL1 leukemia.
Slupianek A, Dasgupta Y, Ren SY, Gurdek E, Donlin M, Nieborowska-Skorska M, Fleury F, Skorski T
Blood 2011 Jul 28;118(4):1062-8
Blood 2011 Jul 28;118(4):1062-8
Selenoprotein K knockout mice exhibit deficient calcium flux in immune cells and impaired immune responses.
Verma S, Hoffmann FW, Kumar M, Huang Z, Roe K, Nguyen-Wu E, Hashimoto AS, Hoffmann PR
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 15;186(4):2127-37
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 15;186(4):2127-37
Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection.
Kadiu I, Gendelman HE
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2011 Dec;6(4):658-75
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2011 Dec;6(4):658-75
MAPK phosphatase-1 contributes to trichostatin A inhibition of cyclooxygenase-2 expression in human umbilical vascular endothelial cells exposed to lipopolysaccharide.
Hsu YF, Sheu JR, Lin CH, Chen WC, Hsiao G, Ou G, Chiu PT, Hsu MJ
Biochimica et biophysica acta 2011 Dec;1810(12):1160-9
Biochimica et biophysica acta 2011 Dec;1810(12):1160-9
Specific antioxidant selenoproteins are induced in the heart during hypertrophy.
Hoffmann FW, Hashimoto AS, Lee BC, Rose AH, Shohet RV, Hoffmann PR
Archives of biochemistry and biophysics 2011 Aug 1;512(1):38-44
Archives of biochemistry and biophysics 2011 Aug 1;512(1):38-44
Cell-cell junctions and vascular endothelial growth factor in rat lung as affected by ischemia/reperfusion and preconditioning with inhaled nitric oxide.
Waldow T, Witt W, Janke A, Ulmer A, Buzin A, Matschke K
The Journal of surgical research 2009 Nov;157(1):30-42
The Journal of surgical research 2009 Nov;157(1):30-42
Glycogen synthase kinase-3/Shaggy mediates ethanol-induced excitotoxic cell death of Drosophila olfactory neurons.
French RL, Heberlein U
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 8;106(49):20924-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 8;106(49):20924-9
Proteomic studies on low- and high-grade human brain astrocytomas.
Odreman F, Vindigni M, Gonzales ML, Niccolini B, Candiano G, Zanotti B, Skrap M, Pizzolitto S, Stanta G, Vindigni A
Journal of proteome research 2005 May-Jun;4(3):698-708
Journal of proteome research 2005 May-Jun;4(3):698-708
G protein binding and G protein activation by nucleotide transfer involve distinct domains on tubulin: regulation of signal transduction by cytoskeletal elements.
Roychowdhury S, Wang N, Rasenick MM
Biochemistry 1993 May 11;32(18):4955-61
Biochemistry 1993 May 11;32(18):4955-61
G protein binding and G protein activation by nucleotide transfer involve distinct domains on tubulin: regulation of signal transduction by cytoskeletal elements.
Roychowdhury S, Wang N, Rasenick MM
Biochemistry 1993 May 11;32(18):4955-61
Biochemistry 1993 May 11;32(18):4955-61
The role of microtubules in platelet secretory release.
Berry S, Dawicki DD, Agarwal KC, Steiner M
Biochimica et biophysica acta 1989 Jun 15;1012(1):46-56
Biochimica et biophysica acta 1989 Jun 15;1012(1):46-56
The role of microtubules in platelet secretory release.
Berry S, Dawicki DD, Agarwal KC, Steiner M
Biochimica et biophysica acta 1989 Jun 15;1012(1):46-56
Biochimica et biophysica acta 1989 Jun 15;1012(1):46-56
Carboxy-terminal regions on the surface of tubulin and microtubules. Epitope locations of YOL1/34, DM1A and DM1B.
Breitling F, Little M
Journal of molecular biology 1986 May 20;189(2):367-70
Journal of molecular biology 1986 May 20;189(2):367-70
Carboxy-terminal regions on the surface of tubulin and microtubules. Epitope locations of YOL1/34, DM1A and DM1B.
Breitling F, Little M
Journal of molecular biology 1986 May 20;189(2):367-70
Journal of molecular biology 1986 May 20;189(2):367-70
10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin.
Blose SH, Meltzer DI, Feramisco JR
The Journal of cell biology 1984 Mar;98(3):847-58
The Journal of cell biology 1984 Mar;98(3):847-58
10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin.
Blose SH, Meltzer DI, Feramisco JR
The Journal of cell biology 1984 Mar;98(3):847-58
The Journal of cell biology 1984 Mar;98(3):847-58
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Western blot analysis of extracts from HeLa, COS and C6 cells using alpha Tubulin antibody (NB100-690, 1:1000, Alpha tubulin molecular weight: 50 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: alpha Tubulin Antibody (DM1A) [NB100-690] - Simple Western lane view shows a specific band for alpha Tubulin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Alpha tubulin molecular weight: 50 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analsis of alpha tubulin in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. RAT2; Lane 7. CHO; Lane 8. MDBK; Lane 9. MDCK
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of alpha tubulin (molecular weight of 50 kDa) in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. RAT2; Lane 7. CHO; Lane 8. MDBK; Lane 9. MDCK
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa and COS-7 lysates. Alpha tubulin molecular weight: 50 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse skeletal muscle using alpha Tubulin Antibody (DM1A) at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong cytoplasmic signal in the muscle cells with cytoplasmic-nuclear signal in the endothelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse lung using alpha Tubulin Antibody (DM1A) at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated chunks of cytoplasmic signal in the alveolar and bronchiolar epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse heart using alpha Tubulin Antibody (DM1A) at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong and specific cytoplasmic signal in the muscle cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of colon tissue. Sections were formalin fixed and embedded with paraffin. Sodium citrate heat mediated antigen retrieval for 20 min. Incubated with primary antibody for 15 min at a 5 ug/ml concentration. Corner image is staining with secondary only.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed colon sections. Heat mediated antigen retrieval was performed using sodium citrate buffer for 20 min before incubating with primary antibody at a 0.5ug/ml dilution for 15 min at RT.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of small intestine tissue fixed with formalin and paraffin embedded showing cytoplasmic and cytoskeletal staining of glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of formalin fixed paraffin embedded heart sections. Used at a dilution of 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of paraffin embedded colon sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse prostate using alpha Tubulin Antibody (DM1A) at 1:200 dilution. The signal was developed using HRP labelled secondary and DAB reagent which followed counterstaining with hematoxylin. The antibody generated a specific cytoplasmic/cytoskeletal staining in the prostate epithelial cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using alpha Tubulin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on SH-SY5Y cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on U-937 cells with alpha Tubulin Antibody (DM1A) NB100-690AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin Antibody (DM1A) NB100-690AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image from the Alexa Fluor 488 version of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690JF646 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Janelia Fluor 646.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Staining of the marine parasite Cryptocaryon irritans mouth. Large bundles of microtubules form a cytophyrigeal basket.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunomicroscopy: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa cells, green staining is alpha tubulin whereas red is DNA stained with propidium iodide.