NB600-506
antibody from Novus Biologicals
Targeting: TUBA1A
B-ALPHA-1, FLJ25113, TUBA3
Antibody data
- Antibody Data
- Antigen structure
- References [34]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Flow cytometry [4]
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Validation data
Reference
Comment
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- Product number
- NB600-506 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-506, RRID:AB_10078394
- Product name
- Rat Monoclonal alpha Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Drosophila, Simian, Yeast
- Host
- Rat
- Antigen sequence
A linear sequence requiring an arom
atic residue at the C terminus, wit
h the two adjacent amino acids bein
g negatively charged (represented b
y Gly-Gly-Tyr in Tyr-Tubulin).- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The Flexiscope: a low cost, flexible, convertible and modular microscope with automated scanning and micromanipulation.
Deficiency in ZMPSTE24 and resulting farnesyl-prelamin A accumulation only modestly affect mouse adipose tissue stores.
Metabolites Produced by the Oral Commensal Bacterium Corynebacterium durum Extend the Lifespan of Caenorhabditis elegans via SIR-2.1 Overexpression.
Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
Protein phosphatase 2A is essential to maintain meiotic arrest, and to prevent Ca2+ burst at spawning and eventual parthenogenesis in the larvacean Oikopleura dioica.
Misplaced Golgi Elements Produce Randomly Oriented Microtubules and Aberrant Cortical Arrays of Microtubules in Dystrophic Skeletal Muscle Fibers.
The conservation and functionality of the oxygen-sensing enzyme Factor Inhibiting HIF (FIH) in non-vertebrates.
Tyrosine Threonine Kinase Inhibition Eliminates Lung Cancers by Augmenting Apoptosis and Polyploidy.
Krt5(+)/Krt15(+) foregut basal progenitors give rise to cyclooxygenase-2-dependent tumours in response to gastric acid stress.
Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant.
VISAGE Reveals a Targetable Mitotic Spindle Vulnerability in Cancer Cells.
Characterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1.
Enhanced susceptibility of cancer cells to oncolytic rhabdo-virotherapy by expression of Nodamura virus protein B2 as a suppressor of RNA interference.
Complexes of α6β4 integrin and vimentin act as signaling hubs to regulate epithelial cell migration.
Delivery of Native Proteins into C. elegans Using a Transduction Protocol Based on Lipid Vesicles.
PLK1-mediated phosphorylation of WDR62/MCPH2 ensures proper mitotic spindle orientation.
111In- and IRDye800CW-Labeled PLA-PEG Nanoparticle for Imaging Prostate-Specific Membrane Antigen-Expressing Tissues.
Genomic Instability Is Induced by Persistent Proliferation of Cells Undergoing Epithelial-to-Mesenchymal Transition.
Specific CP110 Phosphorylation Sites Mediate Anaphase Catastrophe after CDK2 Inhibition: Evidence for Cooperation with USP33 Knockdown.
Proliferative activity of liver growth factor is associated with an improvement of cigarette smoke-induced emphysema in mice.
BRCA1 regulates microtubule dynamics and taxane-induced apoptotic cell signaling.
Cytokinesis failure triggers hippo tumor suppressor pathway activation.
A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony.
Yeast protective response to arsenate involves the repression of the high affinity iron uptake system.
Cells satisfy the mitotic checkpoint in Taxol, and do so faster in concentrations that stabilize syntelic attachments.
Relative contributions of chromatin and kinetochores to mitotic spindle assembly.
Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
Recruitment of vimentin to the cell surface by beta3 integrin and plectin mediates adhesion strength.
A mechanism linking extra centrosomes to chromosomal instability.
Endocytic recycling compartments altered in cisplatin-resistant cancer cells.
Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and G1 tetraploidy checkpoint function.
Extensive contribution of the multidrug transporters P-glycoprotein and Mrp1 to basal drug resistance.
A surface expression vector for antibody screening.
A surface expression vector for antibody screening.
Courtney A, Alvey LM, Merces GOT, Burke N, Pickering M
Royal Society open science 2020 Mar;7(3):191949
Royal Society open science 2020 Mar;7(3):191949
Deficiency in ZMPSTE24 and resulting farnesyl-prelamin A accumulation only modestly affect mouse adipose tissue stores.
Heizer PJ, Yang Y, Tu Y, Kim PH, Chen NY, Hu Y, Yoshinaga Y, de Jong PJ, Vergnes L, Morales JE, Li RL, Jackson N, Reue K, Young SG, Fong LG
Journal of lipid research 2020 Mar;61(3):413-421
Journal of lipid research 2020 Mar;61(3):413-421
Metabolites Produced by the Oral Commensal Bacterium Corynebacterium durum Extend the Lifespan of Caenorhabditis elegans via SIR-2.1 Overexpression.
Kim JH, Bang IH, Noh YJ, Kim DK, Bae EJ, Hwang IH
International journal of molecular sciences 2020 Mar 23;21(6)
International journal of molecular sciences 2020 Mar 23;21(6)
Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
Miyamoto T, Hosoba K, Itabashi T, Iwane AH, Akutsu SN, Ochiai H, Saito Y, Yamamoto T, Matsuura S
The EMBO journal 2020 Jun 17;39(12):e103499
The EMBO journal 2020 Jun 17;39(12):e103499
Protein phosphatase 2A is essential to maintain meiotic arrest, and to prevent Ca2+ burst at spawning and eventual parthenogenesis in the larvacean Oikopleura dioica.
Matsuo M, Onuma TA, Omotezako T, Nishida H
Developmental biology 2020 Apr 15;460(2):155-163
Developmental biology 2020 Apr 15;460(2):155-163
Misplaced Golgi Elements Produce Randomly Oriented Microtubules and Aberrant Cortical Arrays of Microtubules in Dystrophic Skeletal Muscle Fibers.
Oddoux S, Randazzo D, Kenea A, Alonso B, Zaal KJM, Ralston E
Frontiers in cell and developmental biology 2019;7:176
Frontiers in cell and developmental biology 2019;7:176
The conservation and functionality of the oxygen-sensing enzyme Factor Inhibiting HIF (FIH) in non-vertebrates.
Hampton-Smith RJ, Davenport BA, Nagarajan Y, Peet DJ
PloS one 2019;14(4):e0216134
PloS one 2019;14(4):e0216134
Tyrosine Threonine Kinase Inhibition Eliminates Lung Cancers by Augmenting Apoptosis and Polyploidy.
Zheng L, Chen Z, Kawakami M, Chen Y, Roszik J, Mustachio LM, Kurie JM, Villalobos P, Lu W, Behrens C, Mino B, Solis LM, Silvester J, Thu KL, Cescon DW, Rodriguez-Canales J, Wistuba II, Mak TW, Liu X, Dmitrovsky E
Molecular cancer therapeutics 2019 Oct;18(10):1775-1786
Molecular cancer therapeutics 2019 Oct;18(10):1775-1786
Krt5(+)/Krt15(+) foregut basal progenitors give rise to cyclooxygenase-2-dependent tumours in response to gastric acid stress.
Moon H, Zhu J, Donahue LR, Choi E, White AC
Nature communications 2019 May 20;10(1):2225
Nature communications 2019 May 20;10(1):2225
Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant.
Nambiar TS, Billon P, Diedenhofen G, Hayward SB, Taglialatela A, Cai K, Huang JW, Leuzzi G, Cuella-Martin R, Palacios A, Gupta A, Egli D, Ciccia A
Nature communications 2019 Jul 30;10(1):3395
Nature communications 2019 Jul 30;10(1):3395
VISAGE Reveals a Targetable Mitotic Spindle Vulnerability in Cancer Cells.
Patterson JC, Joughin BA, Prota AE, Mühlethaler T, Jonas OH, Whitman MA, Varmeh S, Chen S, Balk SP, Steinmetz MO, Lauffenburger DA, Yaffe MB
Cell systems 2019 Jul 24;9(1):74-92.e8
Cell systems 2019 Jul 24;9(1):74-92.e8
Characterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1.
Kittipassorn T, Haydinger CD, Wood JPM, Mammone T, Casson RJ, Peet DJ
Experimental eye research 2019 Apr;181:127-135
Experimental eye research 2019 Apr;181:127-135
Enhanced susceptibility of cancer cells to oncolytic rhabdo-virotherapy by expression of Nodamura virus protein B2 as a suppressor of RNA interference.
Bastin D, Aitken AS, Pelin A, Pikor LA, Crupi MJF, Huh MS, Bourgeois-Daigneault MC, Bell JC, Ilkow CS
Journal for immunotherapy of cancer 2018 Jun 19;6(1):62
Journal for immunotherapy of cancer 2018 Jun 19;6(1):62
Complexes of α6β4 integrin and vimentin act as signaling hubs to regulate epithelial cell migration.
Colburn ZT, Jones JCR
Journal of cell science 2018 Jul 30;131(14)
Journal of cell science 2018 Jul 30;131(14)
Delivery of Native Proteins into C. elegans Using a Transduction Protocol Based on Lipid Vesicles.
Perni M, Aprile FA, Casford S, Mannini B, Sormanni P, Dobson CM, Vendruscolo M
Scientific reports 2017 Nov 8;7(1):15045
Scientific reports 2017 Nov 8;7(1):15045
PLK1-mediated phosphorylation of WDR62/MCPH2 ensures proper mitotic spindle orientation.
Miyamoto T, Akutsu SN, Fukumitsu A, Morino H, Masatsuna Y, Hosoba K, Kawakami H, Yamamoto T, Shimizu K, Ohashi H, Matsuura S
Human molecular genetics 2017 Nov 15;26(22):4429-4440
Human molecular genetics 2017 Nov 15;26(22):4429-4440
111In- and IRDye800CW-Labeled PLA-PEG Nanoparticle for Imaging Prostate-Specific Membrane Antigen-Expressing Tissues.
Banerjee SR, Foss CA, Horhota A, Pullambhatla M, McDonnell K, Zale S, Pomper MG
Biomacromolecules 2017 Jan 9;18(1):201-209
Biomacromolecules 2017 Jan 9;18(1):201-209
Genomic Instability Is Induced by Persistent Proliferation of Cells Undergoing Epithelial-to-Mesenchymal Transition.
Comaills V, Kabeche L, Morris R, Buisson R, Yu M, Madden MW, LiCausi JA, Boukhali M, Tajima K, Pan S, Aceto N, Sil S, Zheng Y, Sundaresan T, Yae T, Jordan NV, Miyamoto DT, Ting DT, Ramaswamy S, Haas W, Zou L, Haber DA, Maheswaran S
Cell reports 2016 Dec 6;17(10):2632-2647
Cell reports 2016 Dec 6;17(10):2632-2647
Specific CP110 Phosphorylation Sites Mediate Anaphase Catastrophe after CDK2 Inhibition: Evidence for Cooperation with USP33 Knockdown.
Hu S, Lu Y, Orr B, Godek K, Mustachio LM, Kawakami M, Sekula D, Compton DA, Freemantle S, Dmitrovsky E
Molecular cancer therapeutics 2015 Nov;14(11):2576-85
Molecular cancer therapeutics 2015 Nov;14(11):2576-85
Proliferative activity of liver growth factor is associated with an improvement of cigarette smoke-induced emphysema in mice.
Girón-Martínez Á, Pérez-Rial S, Terrón-Expósito R, Díaz-Gil JJ, González-Mangado N, Peces-Barba G
PloS one 2014;9(11):e112995
PloS one 2014;9(11):e112995
BRCA1 regulates microtubule dynamics and taxane-induced apoptotic cell signaling.
Sung M, Giannakakou P
Oncogene 2014 Mar 13;33(11):1418-28
Oncogene 2014 Mar 13;33(11):1418-28
Cytokinesis failure triggers hippo tumor suppressor pathway activation.
Ganem NJ, Cornils H, Chiu SY, O'Rourke KP, Arnaud J, Yimlamai D, Théry M, Camargo FD, Pellman D
Cell 2014 Aug 14;158(4):833-848
Cell 2014 Aug 14;158(4):833-848
A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony.
Whitfield ZJ, Chisholm J, Hawley RS, Orr-Weaver TL
PLoS biology 2013 Sep;11(9):e1001648
PLoS biology 2013 Sep;11(9):e1001648
Yeast protective response to arsenate involves the repression of the high affinity iron uptake system.
Batista-Nascimento L, Toledano MB, Thiele DJ, Rodrigues-Pousada C
Biochimica et biophysica acta 2013 May;1833(5):997-1005
Biochimica et biophysica acta 2013 May;1833(5):997-1005
Cells satisfy the mitotic checkpoint in Taxol, and do so faster in concentrations that stabilize syntelic attachments.
Yang Z, Kenny AE, Brito DA, Rieder CL
The Journal of cell biology 2009 Sep 7;186(5):675-84
The Journal of cell biology 2009 Sep 7;186(5):675-84
Relative contributions of chromatin and kinetochores to mitotic spindle assembly.
O'Connell CB, Loncarek J, Kaláb P, Khodjakov A
The Journal of cell biology 2009 Oct 5;187(1):43-51
The Journal of cell biology 2009 Oct 5;187(1):43-51
Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
Pilling D, Fan T, Huang D, Kaul B, Gomer RH
PloS one 2009 Oct 16;4(10):e7475
PloS one 2009 Oct 16;4(10):e7475
Recruitment of vimentin to the cell surface by beta3 integrin and plectin mediates adhesion strength.
Bhattacharya R, Gonzalez AM, Debiase PJ, Trejo HE, Goldman RD, Flitney FW, Jones JC
Journal of cell science 2009 May 1;122(Pt 9):1390-400
Journal of cell science 2009 May 1;122(Pt 9):1390-400
A mechanism linking extra centrosomes to chromosomal instability.
Ganem NJ, Godinho SA, Pellman D
Nature 2009 Jul 9;460(7252):278-82
Nature 2009 Jul 9;460(7252):278-82
Endocytic recycling compartments altered in cisplatin-resistant cancer cells.
Liang XJ, Mukherjee S, Shen DW, Maxfield FR, Gottesman MM
Cancer research 2006 Feb 15;66(4):2346-53
Cancer research 2006 Feb 15;66(4):2346-53
Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and G1 tetraploidy checkpoint function.
Iida S, Hirota T, Morisaki T, Marumoto T, Hara T, Kuninaka S, Honda S, Kosai K, Kawasuji M, Pallas DC, Saya H
Oncogene 2004 Jul 8;23(31):5266-74
Oncogene 2004 Jul 8;23(31):5266-74
Extensive contribution of the multidrug transporters P-glycoprotein and Mrp1 to basal drug resistance.
Allen JD, Brinkhuis RF, van Deemter L, Wijnholds J, Schinkel AH
Cancer research 2000 Oct 15;60(20):5761-6
Cancer research 2000 Oct 15;60(20):5761-6
A surface expression vector for antibody screening.
Breitling F, Dübel S, Seehaus T, Klewinghaus I, Little M
Gene 1991 Aug 15;104(2):147-53
Gene 1991 Aug 15;104(2):147-53
A surface expression vector for antibody screening.
Breitling F, Dübel S, Seehaus T, Klewinghaus I, Little M
Gene 1991 Aug 15;104(2):147-53
Gene 1991 Aug 15;104(2):147-53
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Total protein from human HeLa, mouse 3T3, rat PC12 and African green monkey Cos7 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:6000 dilution of anti-Tubulin (YL 1/2) in 1% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence. Alpha tubulin molecular weight: 50 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Western Blot analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2). Alpha tubulin molecular weight: 50 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Western blot analysis of alpha Tubulin (molecular weight: 50 kDa) expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tissue levels of VEGF and PCNA.VEGF levels were estimated by western blot. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0112995), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on NIH3T3 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on SH-SY5Y cells with alpha Tubulin (YL1/2) Antibody NB600-506G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-506. An intracellular stain was performed on HeLa cells with Tubulin antibody (YL1/2) NB600-506AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (YL1/2) [NB600-506] - An intracellular stain was performed on PC12 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.