PA5-13467

antibody from Invitrogen Antibodies

Targeting: ACTG1 ACTG, DFNA20, DFNA26

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13467

Provider

Invitrogen Antibodies

Product name

gamma Actin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, chicken, canine, equine, hamster, mouse, porcine, non-human primate, rat, rabbit and Xenopus, yeast and zebrafish based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.

Ouyang X, Dong C, Ubogu EE
Journal of the peripheral nervous system : JPNS 2019 Jun;24(2):195-206

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using an ACTG1 polyclonal antibody (Product # PA5-13467) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of K562 cells using an ACTG1 polyclonal antibody (Product # PA5-13467) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Peripheral nerve cell-specific structural proteins. Representative indirect fluorescent digital photomicrographs of cryostat axial sections of normal adult sural nerves show UEA-1-positive endothelial cells (green; A, E, I, M, Q) with expression of PCDH1, CD44, ACTG1, CALD1, and MYO10 (red; B, F, J, N, R, respectively) on endoneurial microvessels, fenestrated epineurial macrovessels, perineurium and Schwann cells (PCDH1) and axons (CD44, ACTG1, CALD1, and MYO10), shown in the merged images at lower and higher magnifications (yellow/orange for endothelial cells). Expression of these proteins by these cell types implies roles in maintaining the structural integrity of peripheral nerve-specific cells in normal adult nerves. White arrows demonstrate positively staining endoneurial microvessels. Blue (4, 6-diamidino-2-phenylindole) staining indicates nuclei. Scale bar 500 mum for A-C, E-G, I-K, M-O, and Q-S and 125 mum for D, H, L, P, and T