TA325114

antibody from OriGene

Targeting: ZNF202 ZKSCAN10, ZSCAN42

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA325114

Provider

OriGene

Product name

Rabbit polyclonal ZNF202 Antibody (Center)

Antibody type

Polyclonal

Description

Rabbit polyclonal ZNF202 Antibody (Center)

Host

Rabbit

Conjugate

Unconjugated

Epitope

ZNF202

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.48 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of ZNF202 Antibody (Center) (Cat. #TA325114) in HepG2 cell line lysates (35ug/lane). ZNF202 (arrow) was detected using the purified Pab.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of ZNF202 Antibody (Center) (Cat#TA325114) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of MDA-MB231 cell stained with ZNF202 Antibody (Center)(Cat#TA325114). MDA-MB231 cells were incubated with ZNF202 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .ZNF202 immunoreactivity is localized to nucleus significantly.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

ZNF202 Antibody (Center) (Cat. #TA325114) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF202 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

ZNF202 Antibody (Center) (Cat. #TA325114) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Validation comment

FC