66260-1-IG

antibody from Invitrogen Antibodies

Targeting: A1BG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66260-1-IG

Provider

Invitrogen Antibodies

Product name

alpha-1B-glycoprotein Monoclonal Antibody (4D4E5)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MAPVSWITP GLKTTAVCRG VLRGVTFLLR REGDHEFLEV PEAQEDVEAT FPVHQPGNYS CSYRTDGEGA LSEPSATVTI EELAAPPPPV LMHHGESSQV LHPGNKVTLT CVAPLSGVDF QLRRGEKELL VPRSSTSPDR IFFHLNAVAL GDGGHYTCRY RLHDNQNGWS GDSAPVELIL SDETLPAPEF SPEPESGRAL RLRCLAPLEG ARFALVREDR GGRRVHRFQS PAGTEALFEL HNISVADSAN YSCVYVDLKP PFGGSAPSER LELHVDGPPP RPQLRATWSG AVLAGRDAVL RCEGPIPDVT FELLREGETK AVKTVRTPGA AANLELIFVG PQHAGNYRCR YRSWVPHTFE SELSDPVELL VAES (122-495 aa encoded by B C035719)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4D4E5

Vial size

150 µL

Concentration

0.89 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human plasma tissue were subjected to SDS PAGE followed by western blot with 66260-1-IG (alpha-1B-glycoprotein antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human plasma tissue were subjected to SDS PAGE followed by western blot with 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 66260-1-Ig (alpha-1B-glycoprotein antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 66260-1-IG (alpha-1B-glycoprotein antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66260-1-IG (alpha-1B-glycoprotein antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66260-1-IG (alpha-1B-glycoprotein antibody) at dilution of 1:200 (under 40x lens).