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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ8416 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- L3MBTL3 Antibody / Lethal(3)malignant brain tumor-like protein 3
- Antibody type
- Polyclonal
- Description
- This highly specific L3MBTL3 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the L3MBTL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) Jurkat, 2) HEL, 3) 293T and 4) ThP-1 cell lysate with L3MBTL3 antibody. Predicted molecular weight ~88 kDa and ~86 kDa (two isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human bladder urothelial carcinoma tissue with L3MBTL3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human U-251 cells with L3MBTL3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= L3MBTL3 antibody.
