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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-34677 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Plasminogen Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, K562, BCL-1, PC-12, Rat2, human plasma, mouse plasma, rat plasma. Predicted reactivity: Rat (80%), Dog (81%), Pig (80%), Rhesus Monkey (93%), Bovine (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.18 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Schistosoma mansoni phosphoglycerate mutase: a glycolytic ectoenzyme with thrombolytic potential.
Longitudinal proteomics study of serum changes after allogeneic HSCT reveals potential markers of metabolic complications related to aGvHD.
PLG inhibits Hippo signaling pathway through SRC in the hepatitis B virus-induced hepatocellular-carcinoma progression.
Schistosoma mansoni glyceraldehyde-3-phosphate dehydrogenase enhances formation of the blood-clot lysis protein plasmin.
Pirovich DB, Da'dara AA, Skelly PJ
Parasite (Paris, France) 2022;29:41
Parasite (Paris, France) 2022;29:41
Longitudinal proteomics study of serum changes after allogeneic HSCT reveals potential markers of metabolic complications related to aGvHD.
Wong SY, Kato S, Rodenburg F, Tojo A, Hayashi N
Scientific reports 2022 Aug 17;12(1):14002
Scientific reports 2022 Aug 17;12(1):14002
PLG inhibits Hippo signaling pathway through SRC in the hepatitis B virus-induced hepatocellular-carcinoma progression.
Hu ZG, Chen YB, Huang M, Tu JB, Tu SJ, Pan YJ, Chen XL, He SQ
American journal of translational research 2021;13(2):515-531
American journal of translational research 2021;13(2):515-531
Schistosoma mansoni glyceraldehyde-3-phosphate dehydrogenase enhances formation of the blood-clot lysis protein plasmin.
Pirovich DB, Da'dara AA, Skelly PJ
Biology open 2020 Mar 24;9(3)
Biology open 2020 Mar 24;9(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Plasminogen in methanol-fixed HeLa cells using a Plasminogen polyclonal antibody (Product # PA5-34677) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5. Recombinant SmGAPDH binds to plasminogen and enhances plasmin generation. (A) Plasminogen binding to rSmGAPDH (0.5 mug, red line) versus control protein BSA (0.5 mug, dark blue line) detected by ELISA (mean of triplicate OD 450 values+-s.e.m.). Significant difference between plasminogen binding to rSmGAPDH and BSA at all assay points are denoted by *** P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Post-transplant serial changes in four serum proteins from Patient no.1. Serum samples collected over time from patient no.1 were subjected to western blotting analysis. Serum proteins of 10 ug was first separated by 8-12% SDS-PAGE and Precision Plus Protein WesternC Standard (Bio-Rad) was used as protein standard. Proteins were then transferred to a PVDF membrane and stained with antibodies. Primary antibodies were diluted at 2000-fold except for FTL (1000-fold). HRP Goat Anti-Rabbit IgG was used as secondary antibody in 2000-fold dilution except for RBP4 (10,000-fold). Signals of bands were quantified using ImageJ software.
