PA1-600

antibody from Invitrogen Antibodies

Targeting: PLG

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-600

Provider

Invitrogen Antibodies

Product name

Angiostatin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-600 detects recombinant human and mouse angiostatin. PA1-600 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a 50 kDa protein representing recombinant human angiostatin. PA1-600 immunizing peptide corresponds to amino acid residues 98-116 from mouse angiostatin. This sequence is 95% and 84% conserved in rat and human angiostatin, respectively. PA1-600 can be used with blocking peptide PEP-111.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Janus Kinase 1 Plays a Critical Role in Mammary Cancer Progression.

Wehde BL, Rädler PD, Shrestha H, Johnson SJ, Triplett AA, Wagner KU
Cell reports 2018 Nov 20;25(8):2192-2207.e5

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Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin.

Hutzen B, Bid HK, Houghton PJ, Pierson CR, Powell K, Bratasz A, Raffel C, Studebaker AW
BMC cancer 2014 Mar 19;14:206

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Angiostatin (green) showing staining in the cytoplasm of K562 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Angiostatin polyclonal antibody (Product # PA1-600) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Angiostatin (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Angiostatin polyclonal antibody (Product # PA1-600) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 MV-hE:A and MV-mE:A infection results in the secretion of active endostatin:angiostatin. (A) Human endostatin production in infected D283med and D425med cells as quantified by ELISA. Endostatin concentration is expressed in ng/ml per 10 4 cells. (B) Western blot analysis of media taken from either MV-hE:A or MV-mE:A infected D283med and D425med probed with an anti-angiostatin antibody. (C-D) Conditioned media from MV-hE:A and MV-mE:A infected Vero cells (10 mug/ml total protein) inhibits VEGF-mediated tube formation in HUVEC cells (*p