Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-37247 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cyclin E1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mouse mAb against Hela (1), K562 (2), NIH/3T3 (3), C6 (4), MCF-7 (5), Jurkat (6), A431 (7) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mAb against human CCNE1 (AA: 307-410) recombinant protein. (Expected MW is 37.5 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mAb against HEK293 (1) and CCNE1 (AA: 307-410)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Flow cytometric analysis of K652 cells using CCNE1 mouse mAb (green) and negative control (red).