32-1600

antibody from Invitrogen Antibodies

Targeting: CCNE1 CCNE

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

32-1600

Provider

Invitrogen Antibodies

Product name

Cyclin E Monoclonal Antibody (HE12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

HE12

Vial size

200 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

MicroRNA-18a targeting of the STK4/MST1 tumour suppressor is necessary for transformation in HPV positive cervical cancer.

Morgan EL, Patterson MR, Ryder EL, Lee SY, Wasson CW, Harper KL, Li Y, Griffin S, Blair GE, Whitehouse A, Macdonald A
PLoS pathogens 2020 Jun;16(6):e1008624

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A map of protein dynamics during cell-cycle progression and cell-cycle exit.

Gookin S, Min M, Phadke H, Chung M, Moser J, Miller I, Carter D, Spencer SL
PLoS biology 2017 Sep;15(9):e2003268

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Immunohistochemical prediction of lapatinib efficacy in advanced HER2-positive breast cancer patients.

Duchnowska R, Wysocki PJ, Korski K, Czartoryska-Arłukowicz B, Niwińska A, Orlikowska M, Radecka B, Studziński M, Demlova R, Ziółkowska B, Merdalska M, Hajac Ł, Myśliwiec P, Zuziak D, Dębska-Szmich S, Lang I, Foszczyńska-Kłoda M, Karczmarek-Borowska B, Żawrocki A, Kowalczyk A, Biernat W, Jassem J, Central and East European Oncology Group (CEEOG)
Oncotarget 2016 Jan 5;7(1):550-64

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Suppression of apoptosis by PIF1 helicase in human tumor cells.

Gagou ME, Ganesh A, Thompson R, Phear G, Sanders C, Meuth M
Cancer research 2011 Jul 15;71(14):4998-5008

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Neisseria gonorrhoeae infection induces altered amphiregulin processing and release.

Löfmark S, de Klerk N, Aro H
PloS one 2011 Jan 27;6(1):e16369

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A flow cytometry-based screen of nuclear envelope transmembrane proteins identifies NET4/Tmem53 as involved in stress-dependent cell cycle withdrawal.

Korfali N, Srsen V, Waterfall M, Batrakou DG, Pekovic V, Hutchison CJ, Schirmer EC
PloS one 2011 Apr 14;6(4):e18762

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Chemical genetics approach to restoring p27Kip1 reveals novel compounds with antiproliferative activity in prostate cancer cells.

Rico-Bautista E, Yang CC, Lu L, Roth GP, Wolf DA
BMC biology 2010 Dec 23;8:153

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E2F4 and ribonucleotide reductase mediate S-phase arrest in colon cancer cells treated with chlorophyllin.

Chimploy K, Díaz GD, Li Q, Carter O, Dashwood WM, Mathews CK, Williams DE, Bailey GS, Dashwood RH
International journal of cancer 2009 Nov 1;125(9):2086-94

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Molecular classification and prognostication of adrenocortical tumors by transcriptome profiling.

Giordano TJ, Kuick R, Else T, Gauger PG, Vinco M, Bauersfeld J, Sanders D, Thomas DG, Doherty G, Hammer G
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 15;15(2):668-76

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Differential apoptosis by gallotannin in human colon cancer cells with distinct p53 status.

Al-Ayyoubi S, Gali-Muhtasib H
Molecular carcinogenesis 2007 Mar;46(3):176-86

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Neisseria gonorrhoeae infection causes a G1 arrest in human epithelial cells.

Jones A, Jonsson AB, Aro H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Feb;21(2):345-55

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The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cells.

Lu L, Schulz H, Wolf DA
BMC cell biology 2002 Aug 20;3:22

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The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cells.

Lu L, Schulz H, Wolf DA
BMC cell biology 2002 Aug 20;3:22

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cyclin E was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Cyclin E Mouse Monoclonal Antibody (Product # 32-1600) at 2 µg - 4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (Product # A12381). Panel d is a merged image showing nuclear localization of Cyclin E. Panel e shows no primary antibody. The images were captured at 20X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Cyclin E was done on synchronized HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were labeled with Cyclin E Mouse Monoclonal Antibody (321600, red histogram) or with mouse isotype control (pink histogram) at 2.5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 - 3 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 9 NET4/TMEM53 knockdown did not affect levels of other nuclear envelope proteins linked to the cell cycle or cyclins. (A) Protein lysates were recovered from U2OS cells treated as in Figure 8 and reacted on Western blots with antibodies to the various proteins. This experiment was repeated 3 times and a representative Western blot is shown. (B) Cyclin levels were quantified from three separate experiments analyzed by LI-COR using fluorescent secondary antibodies and are plotted normalized to the actin control. Standard errors are shown.