Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A00543-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cyclin E1/CCNE1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Cyclin E1/CCNE1 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter.
Puerarin prevents progression of experimental hypoxia-induced pulmonary hypertension via inhibition of autophagy.
Long noncoding RNA Hoxaas3 contributes to hypoxia-induced pulmonary artery smooth muscle cell proliferation.
Down-regulation of long non-coding RNA MALAT1 by RNA interference inhibits proliferation and induces apoptosis in multiple myeloma.
Silencing of astrocyte elevated gene-1 inhibits proliferation and migration of melanoma cells and induces apoptosis.
Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 oncogene.
Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro.
ECRG4 as a novel tumor suppressor gene inhibits colorectal cancer cell growth in vitro and in vivo.
Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins.
A role for LRIG1 in the regulation of malignant glioma aggressiveness.
The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells.
RhoC regulates the proliferation of gastric cancer cells through interaction with IQGAP1.
Anticancer effect of Lycium barbarum polysaccharides on colon cancer cells involves G0/G1 phase arrest.
Growth inhibition and cell-cycle arrest of human gastric cancer cells by Lycium barbarum polysaccharide.
Effect of total saponins of "panax notoginseng root" on aortic intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix in rats.
Crocetin inhibits cell cycle G1/S transition through suppressing cyclin D1 and elevating p27kip1 in vascular smooth muscle cells.
Specific COX-2 inhibitor, meloxicam, suppresses proliferation and induces apoptosis in human HepG2 hepatocellular carcinoma cells.
Bladder epithelial cell proliferation of rats induced by terephthalic acid-calculi.
Zhang J, Yang Y, Dong Y, Liu C
International journal of molecular medicine 2022 Feb;49(2)
International journal of molecular medicine 2022 Feb;49(2)
Puerarin prevents progression of experimental hypoxia-induced pulmonary hypertension via inhibition of autophagy.
Zhang X, Liu Q, Zhang C, Sheng J, Li S, Li W, Yang X, Wang X, He S, Bai J, Zhu D
Journal of pharmacological sciences 2019 Oct;141(2):97-105
Journal of pharmacological sciences 2019 Oct;141(2):97-105
Long noncoding RNA Hoxaas3 contributes to hypoxia-induced pulmonary artery smooth muscle cell proliferation.
Zhang H, Liu Y, Yan L, Wang S, Zhang M, Ma C, Zheng X, Chen H, Zhu D
Cardiovascular research 2019 Mar 1;115(3):647-657
Cardiovascular research 2019 Mar 1;115(3):647-657
Down-regulation of long non-coding RNA MALAT1 by RNA interference inhibits proliferation and induces apoptosis in multiple myeloma.
Liu H, Wang H, Wu B, Yao K, Liao A, Miao M, Li Y, Yang W
Clinical and experimental pharmacology & physiology 2017 Oct;44(10):1032-1041
Clinical and experimental pharmacology & physiology 2017 Oct;44(10):1032-1041
Silencing of astrocyte elevated gene-1 inhibits proliferation and migration of melanoma cells and induces apoptosis.
Zhang Y, Peng G, Wang Y, Cui L, Wu W, Wang L, Liu C, Han X
Clinical and experimental pharmacology & physiology 2017 Jul;44(7):815-826
Clinical and experimental pharmacology & physiology 2017 Jul;44(7):815-826
Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 oncogene.
Kong Y, Lu ZL, Wang JJ, Zhou R, Guo J, Liu J, Sun HL, Wang H, Song W, Yang J, Xu HX
Oncology reports 2016 Sep;36(3):1447-56
Oncology reports 2016 Sep;36(3):1447-56
Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro.
Yang JB, Khan M, He YY, Yao M, Li YM, Gao HW, Ma TH
Acta pharmacologica Sinica 2016 Jul;37(7):950-62
Acta pharmacologica Sinica 2016 Jul;37(7):950-62
ECRG4 as a novel tumor suppressor gene inhibits colorectal cancer cell growth in vitro and in vivo.
Cai Z, Liang P, Xuan J, Wan J, Guo H
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jul;37(7):9111-20
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jul;37(7):9111-20
Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins.
Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X
Biochemical and biophysical research communications 2013 Oct 18;440(2):322-9
Biochemical and biophysical research communications 2013 Oct 18;440(2):322-9
A role for LRIG1 in the regulation of malignant glioma aggressiveness.
Mao F, Wang B, Xiao Q, Xi G, Sun W, Zhang H, Ye F, Wan F, Guo D, Lei T, Chen X
International journal of oncology 2013 Mar;42(3):1081-7
International journal of oncology 2013 Mar;42(3):1081-7
The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells.
Xu F, You X, Liu F, Shen X, Yao Y, Ye L, Zhang X
Cancer letters 2013 Jun 1;333(1):124-32
Cancer letters 2013 Jun 1;333(1):124-32
RhoC regulates the proliferation of gastric cancer cells through interaction with IQGAP1.
Wu Y, Tao Y, Chen Y, Xu W
PloS one 2012;7(11):e48917
PloS one 2012;7(11):e48917
Anticancer effect of Lycium barbarum polysaccharides on colon cancer cells involves G0/G1 phase arrest.
Mao F, Xiao B, Jiang Z, Zhao J, Huang X, Guo J
Medical oncology (Northwood, London, England) 2011 Mar;28(1):121-6
Medical oncology (Northwood, London, England) 2011 Mar;28(1):121-6
Growth inhibition and cell-cycle arrest of human gastric cancer cells by Lycium barbarum polysaccharide.
Miao Y, Xiao B, Jiang Z, Guo Y, Mao F, Zhao J, Huang X, Guo J
Medical oncology (Northwood, London, England) 2010 Sep;27(3):785-90
Medical oncology (Northwood, London, England) 2010 Sep;27(3):785-90
Effect of total saponins of "panax notoginseng root" on aortic intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix in rats.
Wu L, Zhang W, Tang YH, Li H, Chen BY, Zhang GM, Deng CQ
Phytomedicine : international journal of phytotherapy and phytopharmacology 2010 Mar;17(3-4):233-40
Phytomedicine : international journal of phytotherapy and phytopharmacology 2010 Mar;17(3-4):233-40
Crocetin inhibits cell cycle G1/S transition through suppressing cyclin D1 and elevating p27kip1 in vascular smooth muscle cells.
Zhou CH, Xiang M, He SY, Qian ZY
Phytotherapy research : PTR 2010 Jul;24(7):975-81
Phytotherapy research : PTR 2010 Jul;24(7):975-81
Specific COX-2 inhibitor, meloxicam, suppresses proliferation and induces apoptosis in human HepG2 hepatocellular carcinoma cells.
Li J, Chen X, Dong X, Xu Z, Jiang H, Sun X
Journal of gastroenterology and hepatology 2006 Dec;21(12):1814-20
Journal of gastroenterology and hepatology 2006 Dec;21(12):1814-20
Bladder epithelial cell proliferation of rats induced by terephthalic acid-calculi.
Dai G, Cui L, Song L, Cheng J, Zhong Y, Zhao R, Wang X
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2005 Feb;43(2):217-24
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2005 Feb;43(2):217-24
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of CCNE1 using anti-CCNE1 antibody (A00543-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PANC-1 whole cell lysates, Lane 7: human CACO-2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNE1 antigen affinity purified polyclonal antibody (Catalog # A00543-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNE1 at approximately 47KD. The expected band size for CCNE1 is at 47KD.
- Additional image