APRab09595

antibody from EnkiLife Biotech Co., Ltd.

Targeting: CCNE1 CCNE

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

APRab09595

Provider

EnkiLife Biotech Co., Ltd.

Product name

Cyclin E1 Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Affinity purification

Reactivity

Human, Mouse, Rat, Other

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

Polyclonal

Vial size

100 µl

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Western blot analysis of lysates from K562 cells, using Cyclin E1 Antibody. The lane on the right is blocked with the synthesized peptide.

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Western Blot analysis of various cells using Cyclin E1 Polyclonal Antibody diluted at 1：500

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Western Blot analysis of MCF7 cells using Cyclin E1 Polyclonal Antibody diluted at 1：500

Immunocytochemistry

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of A549 cells, using Cyclin E1 Antibody. The picture on the right is blocked with the synthesized peptide.

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescence analysis of rat-spleen tissue. 1,Cyclin E1 Polyclonal Antibody（red） was diluted at 1:200（4°,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemistry

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Cyclin E1 Antibody. The picture on the right is blocked with the synthesized peptide.

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.