Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF2078 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LILRB2/CD85d/ILT4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LILRB2/CD85d/ILT4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross-reactivity with recombinant human (rh) ILT2 is observed and 5% cross-reactivity with rhILT5 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8N423
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-10 inhibits endothelium-dependent T cell costimulation by up-regulation of ILT3/4 in human vascular endothelial cells.
Gleissner CA, Zastrow A, Klingenberg R, Kluger MS, Konstandin M, Celik S, Haemmerling S, Shankar V, Giese T, Katus HA, Dengler TJ
European journal of immunology 2007 Jan;37(1):177-92
European journal of immunology 2007 Jan;37(1):177-92
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LILRB2/CD85d/ILT4 in Human Lung. LILRB2/CD85d/ILT4 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human LILRB2/CD85d/ILT4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2078) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by LILRB2/CD85d/ILT4 and Neutralization by Human LILRB2/CD85d/ILT4 Antibody. Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (35 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LILRB2/CD85d/ILT4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2078). The ND50 is typically 0.3-1.0 µg/mL.